大叶黄杨抗白粉病生理生化指标的研究 摘 要：以大叶黄杨为研究对象，本实验通过研究大叶黄杨感染白粉病前后，叶绿素含量、过氧化物酶（POD）、超氧化物歧化酶（SOD）活性的变化以及逆境生理指标丙二醛含量的变化和脯氨酸含量的变化，显现出白粉病对植物生长的过程中生理生化指标的影响。结果表明：大叶黄杨发病前后叶绿素含量有所降低，其中大叶黄杨染病后叶绿素含量下降的程度最大，而SOD活性和POD活性，丙二醛含量增高，脯氨酸含量通过制作标准曲线查出测定液中脯氨酸浓度，计算出脯氨酸的含量，得出结果是发病前脯氨酸的含量比发病后脯氨酸含量低。
关键词：大叶黄杨；白粉病 ；生理生化
Euonymus Resistance to Powdery Mildew Physiological Biochemical Index
Abstract: Euonymus japonicus for the study, before and after experimental through research Euonymus infection powdery mildew, chlorophyll content, peroxidase (POD), superoxide dismutase (SOD) activity changes and stress physiology indicators malonicthe aldehyde content changes and proline content changes, showing powdery mildew on physiological and biochemical indexes in the process of plant growth. The results showed that: Euonymus japonicus before and after the onset of chlorophyll content and POD activity decreased, which chlorophyll content decreased Euonymus japonicus infected greatest extent, the activity of SOD, MDA content increased, proline content detected by standard curve Determination of liquid proline concentration, calculated proline, the outcome before the onset of proline content than after the onset of low proline content.
Key Words: Euonymus; Powdery mildew; Physiological and biochemical
目 录
摘要 1
引言 1
1． 材料和方法 2
1.1材料采集 2
1.2实验器材 2
1.3实验试剂 2
2.实验方法 3
2.1叶绿素的制备及含量测定 3
2.2 SOD粗酶提取液的制备及测定 3
2.3 POD粗酶提取液的制备与测定 3
2.4丙二醛含量测定 4
2.5脯氨酸含量的测定 4
2.5.1标准曲线的绘制 4
3.结果 5
3.1白粉病叶片和健康叶片叶绿素含量的比较 5
3.2 SOD活力的测定结果 5
3.3 POD含量测定结果 5
3.4丙二醛含量测定结果 6
3.5脯氨酸含量测定结果 6
4 结果分析 7
参考文献 8
致谢 10，3110