摘要生物催化技术是目前工业可持续发展中最引人注目的技术，相比传统的化学催化有着得天独厚的优势。但是由于游离酶的存活周期往往很短暂，大大限制了其在工业生产的适用空间。因此如何提高酶催化剂在工业催化过程中的稳定性和效率成为了当今生物催化领域的关键课题。88198

本论文以乙醇脱氢酶和醛酮还原酶为原料，通过实验探究了酶晶体的制备和催化2,6-二氯-3-氟苯乙酮的效率。与游离酶相比，固定化酶晶体相对于游离酶催化效率和稳定性都得到了提高。活性物质在多相催化反应中易于再利用，同时在生产实践可以使生产过程实现自动化，持续，而且无酶残留现象。可使后期产品分离纯化工作大大减少甚至避免，从而提高生产效率和节能。本课题使用的方法具有以上操作简单、绿色环保、酶的催化效率和稳定性高等优点。

Biocatalytic technology is currently the most striking technology in industrial sustainable development, compared to the traditional chemical catalysis has a unique advantage。 However, because the free enzyme survival cycle is often very short, greatly limits its application in the industrial space。 Therefore, how to improve the stability and efficiency of the enzyme catalyst in the industrial catalysis has become a key issue in the field of biocatalysis。

In this paper, ethanol dehydrogenase and aldehydes and ketones reductase as raw materials, through the experimental study of the preparation of enzyme crystals and catalytic efficiency。 Compared with the free enzyme, immobilized enzyme crystals relative to the free enzyme catalytic efficiency and stability have been improved。 The active substance is easy to reusable in the heterogeneous catalytic reaction, and the production process can be automated, sustained, and non-enzymatic to the production process。 So that the latter part of the product separation and purification work greatly reduced or even avoided, thereby enhancing production efficiency and energy saving。 The method used in this subject has the advantages of simple operation, green environmental protection, enzyme catalytic efficiency and high stability。

毕业论文关键词：生物催化；固定化；酶晶体；绿色

Keyword: Bio catalysis；immobilization；Enzyme crystals；green

目    录

引言 4

   1。实验背景源-于,优W尔Y论L文.网wwW.youeRw.com 原文+QQ75201,8766概述6

   2。研究目的7

   3。实验部分8

        3。1 主要实验试剂和仪器·8

            3。1。1 实验仪器·8

            3。1。2 实验试剂·8

        3。2 实验过程·9

3。2。1 酶的制备·9

            3。2。2 酶晶体的制备·10

            3。2。3 酶晶体的催化·12

3。2。4 各种条件对催化效率的探究·12

3。2。5 反应介质对催化效率的探究·13

            3。2。6 反应温度From~优E尔L论E文W网wWw.YoUeRw.com 加QQ7520.18766对催化效率的探究·13

            3。2。7 反应时间对催化效率的探究·13

        3。3 微量表征·13

            3。3。1 液相·13

3。3。2 解析·15

   4。总结16

        4。1 探究结果讨论·16

        4。2 实验总结·16

参考文献·18