摘要酶传感器具有高度的选择性，但往往受到修饰过程复杂、修饰产物不稳定的限制，且对环境要求较高，因此促进了对更为敏感、稳定的非酶电化学传感器的开发。本文对一种以卟啉分子为重要原料的新型电致化学发光体的制备和合成进行了研究和检测，该发光体具备卟啉分子优良的光电性能，以及足球烯分子带来的高效电子传输能力。该种发光体被应用于H7N9病毒亚基基因的捕获探针的检测。我们利用环糊精分子的中空桶状结构，将电化学发光体组装并固定在电极表面，以此建立检测该基因的电致化学发光生物传感体系。该体系使用通用、简易的信号标记、放大手段和DNA检测策略，对核酸的灵敏度高、重现性好、检测下限低、线性范围宽、稳定性良好，具有广阔的应用前景。28426
关键词  生物传感器  分子诊断  电致化学发光  卟啉
毕业论文设计说明书外文摘要
Title  Electrochemiluminescence Nuclear Acid Molecular Diagnostics Based  Nanoclusters Probe                                   
Abstract
Although the Enzymatic sensors has a broad selectivity, under the constraints of unstable or complicated modified procedures and demanding experimental conditions, which stimulate the development of the non-enzymatic electrochemical sensors, which are more stable and sensitive. A novel focus on the preparation and testing methods of a newly lumiophore(C60-ZnTPP), which is made from Porphyrin and Fullerenes molecules as the raw materials, it shares excellent optical and electrical properties from Porphyrin and great electronic transmission ability from Fullerenes. One of the non-enzymatic electrochemical sensors has already been successfully applied to the detection of H7N9 virus subunit genes as the capture  probe. By using the Cyclodextrin molecule’s unique barrel structure, we have been able to assemble and fix the electrongenerated chemiluminescence(ECL) onto the surface of electrode and build a ECL biosensor system to detect the virus subunit genes.  With more general and simplified labeling and DNA detection strategy, such system has a great nucleic sensitivity, good reproducibility, low detection limit, broad linear range and excellent stability, which indicates that non-enzymatic sensors have favorable application foreground. 
Keywords  Biosensor  Molecular Diagnostics  Electrochemiluminescence  Porphyrin
目   次
1 引言    1
1.1  卟啉及其应用    1
1.2  ECL电致化学发光技术简介    2
1.3  循环伏安法（CV）简介    4
1.4  研究课题的提出和内容    5
2  实验材料与方法    7
2.1  实验材料    7
2.2  实验方法    8
3  结果与讨论    13
3.1  新型电化学发光体C60-ZnTPP的表征    13
3.2  ECL体系主−客体组装的表征    13
3.3  电致发光DNA传感器的检测    14
3.4  C60−ZnTPP的光谱学特性    15
3.5  ECL体系发光稳定性分析    16
结  论    18
致  谢    19
参考文献20
1 引言
1.1  卟啉及其应用
1.1.1  卟啉的结构及基本性质
卟啉（porphyrin），以卟吩为前体，大分子杂环化合物，卟啉分子通过四个吡咯类亚上的α-碳以基次甲基桥（=CH-）相连得到。卟啉显深色，其颜色来源于环上有6个高度共轭的π电子。本实验使用的间四苯基卟啉锌（ZnTPP），其分子式参见图1.1 。 基于纳米团簇探针的电致发光核酸分子诊断:http://www.youerw.com/huaxue/lunwen_23267.html