摘要：当前重金属污染严重威胁着人类的健康，其中汞离子是危害最大的重金属离子之一，因此研发出有效检测汞离子的探针分子具有重要意义。本文以8-羟基喹啉、烯丙基溴为原料，经过醚化、裂解断键等反应合成目标分子探针H1，并通过1H-NMR，MS图谱表征确认其结构，产率为40%。通过加入5倍汞离子后检测其荧光性能，静置10min后，体系的荧光强度增强约5倍；静置5h之后，荧光强度增强约70倍，此后随时间的增加荧光强度不再改变，说明反应体系趋于稳定。同时通过荧光光谱检测发现，其它金属阳离子对目标探针与汞离子的反应无干扰，最后经核磁滴定发现，H1与汞离子作用后生成了新的化合物Hg-H1。30022
毕业论文关键词： 荧光探针；汞离子识别；8-羟基喹啉；汞诱导反应
New specific recognition based on 8 - hydroxyquinoline mercury ion probe synthesis of molecules and their actual detection applications
Abstract: Currently, heavy metal pollution seriously threatens human health. Mercury ion is one of the most dangerous heavy metal ions. Thereby the development of probe molecules which can effectively detect mercury ion has important significance. In this paper, through etherification, cleavage bond breaking reaction and other reactions, the raw materials 8-hydroxyquinoline and ally bromide were used to synthesize a new type of molecular probe H1, whose yield was 40%. By 1H-NMR, MS spectra the structure was confirmed. 5-fold mercury ions were added and then the fluorescence properties were detected. The fluorescence intensity of the system enhanced about five times after standing 10min; the fluorescence intensity increased about 70 times after standing 5h, the fluorescence intensity did not vary with the increase of the time, which indicating the reaction system stabilized. Meanwhile detected fluorescence spectroscopy showed other metal cations did not interference the reaction of mercury ions with the target probe. We finally found that the probe H1 and mercury ions generated the new compound Hg-H1 by 1H-NMR titration.
Keywords: Fluorescent probe; Mercury ion recognition; 8-Hydroxyquinoline; Mercury induced reaction
目录
1. 绪论 1
1.1 引言 1
1.2 荧光的发光原理 1
1.3 荧光分析法的发展进程 2
1.4 荧光分子探针的概述 2
1.5 汞离子荧光探针的研究现状及分类 3
1.5.1 配位型的汞离子探针 3
1.5.2 反应型的汞离子探针 4
1.6 8-羟基喹啉类荧光分子探针的研究现状 6
1.7 研究目的和意义 6
1.7.1 研究意义 6
1.7.2 研究方案 6
2. 实验部分 8
2.1 实验设备与仪器 8
2.2.1 实验设备 8
2.2.2 实验试剂 8
2.3 实验步骤 9
2.3.1中间体L1的合成 9
2.3.2目标探针分子H1的合成 9
2.4 探针H1的荧光光谱测试方法 10
3 实验结果与讨论 11
3.1探针H1对铁离子的识别性能研究 11
3.1.1探针对汞离子的的选择性测试 11
3.1.2 探针对汞离子的阳离子干扰实验测试 12
3.1.3 探针对汞离子的定量测试 13
3.1.4 探针对汞离子的pH响应测试 14
3.1.5 探针对汞离子的时间响应测试 15
3.2 探针H1对汞离子反应识别机理研究 17 基于8-羟基喹啉的新型专一识别汞离子的分子探合成及其实际检测应用:http://www.youerw.com/huaxue/lunwen_25514.html