摘要本研究采用微生物法降解水中的三乙胺。一种高效的三乙胺降解菌T12从污染土壤中分离得到,经过对T12的生理生化指标的分析及16SrDNA序列的测定、同源性比较分析,最终鉴定T12为索氏菌属 (Thauera sp.),为杆状革兰氏染色阴性菌。并且得到其序列号,生长发育树。通过对生长因子的研究,菌株T12为专性好氧菌,大通气量有利其生长,最适生长温度为30℃,最适pH为7.5,最适含盐量为10g/L,最适三乙胺含量80mg/L。降解菌对碳源的利用,以乙酸钠最好,而对于多数氮源降解菌大都可以利用。通过对环境因素的研究,T12降解三乙胺的适宜条件总体看来不高,对环境条件要求不是十分苛刻。59867
毕业论文关键词 三乙胺 生物降解 索氏菌T12 影响因子
毕业设计说明书(论文)外文摘要
Title Isolation, identification and biological characteristics research of triethylamine degrading bacteria
Abstract
This study used microbiological method to degrade triethylamine. An efficient triethylamine degrading bacteria called T12 was isolated from polluted soil. Through the analysis of the physiological and biochemical indicators of T12, the determination of 16SrDNA sequences and homology comparison analysis, the final identification of T12 is Thauera sp. T12 is rod Gram negative bacteria.We also got the serial number and growth of the tree. Through the research of growth factor, strain T12 is obligate aerobic bacterium, chase volume is good for their growth. The optimum growth temperature is 30 ℃, the optimum pH is 7.5, the optimum salinity is 10 g/L, the optimum content of triethylamine is 80mg/L.Sodium acetate can make great contribution to the growth of T12, while most nitrogen source can be used. Through the study of environmental factors, the environment condition to the triethylamine degrading bacteria is not very demanding.
Keywords triethylamine biodegradation Thauera sp.12 Impact factor
1 引言 1
1.1 环境污染 1
1.2 有机物污染处理技术 1
1.3 生物降解的研究进展 2
1.3.1 影响微生物降解的因素 2
1.3.2 有机物生物降解的机理 2
1.3.3 有机物生物降解途径 3
1.3.4 生物降解研究的发展趋势 4
1.3.5 微生物的净化作用 4
1.4 三乙胺及其降解 5
1.4.1 三乙胺的理化性质及用途 5
1.4.2 三乙胺的毒性和危害 6
1.4.3 三乙胺的相关环境标准 7
1.4.4 三乙胺废水的处理现状 7
1.5 研究目的和内容 9
1.5.1 研究目的和意义 9
1.5.2 研究内容 9
2 三乙胺降解菌株的分离与鉴定 三乙胺降解菌的分离鉴定和生物特性的研究:http://www.youerw.com/huaxue/lunwen_65209.html