铜胁迫引起海州香薷和野草香氧化损伤的差异比较
时间:2018-08-26 19:54 来源:毕业论文 作者:毕业论文 点击:次
| 摘要：采用溶液培养的方法研究了铜胁迫下海州香薷和野草香的地上部和根系的氧化损伤的差异。对MDA、H2O2和O2•-进行了定量和定性检测，并检测了质膜完整性和几种抗氧化酶的活性。结果表明，铜处理条件下海州香薷根系中MDA含量无显著变化，而野草香根系中MDA含量则显著增加；25µM铜处理能诱导海州香薷叶片和根系中H2O2含量增加，对野草香无影响；50µM铜处理能显著增加野草香根系H2O2含量；铜胁迫能显著增加野草香叶片和根系中的O2•-含量，而海州香薷根系在50µM铜处理时才略有增加；铜胁迫能显著破坏野草香根系的质膜完整性，对海州香薷无影响；25µM铜处理时两种植物的抗氧化酶活性水平差距不大，但浓度升高后，海州香薷的抗氧化酶系统更加稳定。27424 毕业论文关键词：铜胁迫；海州香薷；野草香；氧化损伤；抗氧化酶 Comparative Studies of Oxidative Damage between Elsholtzia haichowensis and Elsholtzia cyp riani under Copper Stress Abstract：Hydroponic cultures were utilized to study the difference of oxidative damage in the shoot and root between Elsholtzia haichowensis and Elsholtzia cypriani under copper(Cu) stress. Quantitative and qualitative experiments of MDA、H2O2 and O2•- was conducted. Plasma membrane integrity and activity of several antioxidative enzymes were also investigated. The results showed that, Cu treatments caused significant increases of malondialdehyde (MDA) concentration in the roots of E. cypriani, while had no significant effect on those in the roots of E. haichowensis. 25µM Cu treatment can induced increase of H2O2 concentration in the shoot and root of E. haichowensis, while had no impact on E. cypriani. 50µM copper treatment can significantly increase H2O2 concentration in the root of E. cypriani. Copper stress can significantly increase O2•- concentration in the shoot and root of E. cypriani, while only 50µM copper treatment can slightly increase O2•- concentration in the root of E. haichowensis. Compared with E. haichowensis, copper stress significantly damaged plasma membrane integrity in the root of E.cypriani. There is no difference in the activity of antioxidase between the two plants under copper with concentration of 25µM, however, when copper concentration increased the antioxidant enzyme systems of E. haichowensis were more stable. Key words: Copper Stress; Elsholtzia haichowensis; Elsholtzia cypriani; Oxidative Damage; Antioxidase 目 录 摘要1 关键词1 Abstract1 Key words1 引言1 1材料与方法2 1.1实验材料 2 1.2 实验设计与处理 2 1.3测定方法 3 1.3.1 H2O2 的积累组织化学检测3 1.3.2 O2•-积累的组织化学检测3 1.3.3膜脂过氧化的组织化学检测3 1.3.4 H2O2含量的测定3 1.3.5 O2•-产生速率的测定3 1.3.6 TBARS含量的测定3 1.3.7 质膜完整性的测定3 1.3.8 抗氧化酶活性的测定4 1.3.9 数据处理4 2结果与分析4 2.1铜胁迫对两种植物的生长的影响 4 2.2铜胁迫对两种植物造成氧化损伤的定性检测分析5 2.3铜胁迫对两种植物造成氧化损伤的定量检测分析5 2.3.1 Cu对膜脂过氧化的影响5 2.3.2 Cu对H2O2含量的影响6 2.3.3 Cu对O2•-含量的影响6 2.3.4 Cu对质膜完整性的影响7 2.4铜胁迫对两种植物抗氧化酶活性的影响7 2.4.1 Cu对SOD活力的影响7 2.4.2 Cu对POD活力的影响7 2.4.3 Cu对CAT活力的影响8 3讨论8 3.1铜胁迫对海州香薷和野草香造成的氧化损伤 8 3.2抗氧化酶在海州香薷和野草香的铜耐性中的作用9 3.3海州香薷作为土壤修复植物的应用潜力9 参考文献10 (责任编辑：qin) |