基于SSU rRNA基因序列多环芳烃污染底泥细菌多样性研究
时间:2019-09-17 19:52 来源:毕业论文 作者:毕业论文 点击:次
| 基于SSU rRNA基因序列多环芳烃污染底泥细菌多样性研究摘要:土壤多环芳烃污染的治理、生物降解多环芳烃,可能是当今最重要的生物技术过程之一。通过对多环芳烃污染底泥中的微生物群落结构的分析可能有助于土壤多环芳烃污染治理的研究。在本文中,通过宏基因组方法，我们发现在多环芳烃污染的底泥中最主要的细菌是BetaProteobacteria。从底泥样品中我们获得了25710 个SSU rRNA基因读取,其中41.91%是变形菌门,包括AlphaProteobacteria(3.14%)、BetaProteobacteria(16.73%)、DeltaProteobacteria(8.80%)、EpsilonProteobacteria(2.28%)和GammaProteobacteria(10.72%)。我们还发现,基于16s rRNA基因序列的物种分类表明在多环芳烃污染的底泥中，约41.95%,14.57%和12.84%分别隶属于Betaproteobacteria, Gammaproteobracteria,和Deltaproteobracteria菌种。本文表明,基于SSU rRNA基因序列物种分类分析是一个非常重要的分子生物学方法，它可以快速分离与鉴定土壤中不同的菌种，也是一个重要的分类工具。此外,宏基因组数据显示BetaProteobacteria菌种可能是一个潜在的多环芳烃生物降解细菌，期望在以后可以被用来治理土壤中的多环芳烃污染以及加入生物反应器中增强生物反应器的生物降解能力。39130 毕业论文关键词：土壤宏基因组学 土壤多环芳烃污染 BetaProteobacteria 生物降解 Analysis based by SSU rRNA gene sequence of bacterial persity in PAHs-contaminated bottom mud Abstract: The treatment of polycyclic aromatic hydrocarbons (PAHs) pollution, in terms of biodegrading PAHs, probably today’s most important biotechnological process. The knowledge of bacterial persity in PAHs-contaminated bottom mud could perform great value in treatment of PAHs polluted soil. In this article, we found the most dominant phyla are BetaProteobacteria in PAHs-contaminated bottom mud with metagenomic methods. We got 25710 SSU rRNA gene reads from our sample, 41.91% of which were Proteobacteria with SSU rRNA gene sequence analysis, including AlphaProteobacteria(3.14%), BetaProteobacteria(16.73%), DeltaProteobacteria(8.80%), EpsilonProteobacteria(2.28%) and GammaProteobacteria(10.72%). We also found that the taxonomy based by 16s rRNA gene sequence of this PAHs-contaminated soil microorganisms showed us that about 41.95%, 14.57% and 12.84% were respectively affiliated to class Betaproteobacteria, Gammaproteobracteria, Deltaproteobracteria. This study indicated that SSU rRNA gene sequence analysis was a very important molecular biological method to rapidly screen and identify many different strains in soil, and also a significant tool for classification. Consequently, the metagenomic data showed us the BetaProteobacteria group could be a potential polycyclic aromatic hydrocarbons (PAHs)-degrading bacteria to treat the soil of a multiple contamination by PAHs in the future. It also could be applied in enhancing degradative capacity of bioreactor for treating contaminated soil。 Keywords: Metagenome Polycyclic aromatic hydrocarbons (PAHs) BetaProteobacteria Biodegradation 目录 引言： 4 1 材料与方法： 5 1.1 宏基因组分析 5 1.1.1 底泥样品采集 5 1.1.2 DNA的提取 5 1.1.3 Illumina sequence analysis 5 1.2 基于SSU rRNA基因序列的物种分类 6 1.3 基于16s rRNA基因序列的物种分类 6 2 结果与分析 6 3结论 10 致谢 11 参考文献 11 引言： 多环芳烃是疏水芳香族化合物,由两个或两个以上的苯环或五环以线性排列，角状排列或者稠合多苯结构组成[4]。它们是普遍存在的环境污染物[12]。它们总是以复杂的混合物形式存在于多环芳烃污染的土壤中,许多从多环芳烃污染的土壤中分离的微生物已经被发现可以生长在多环芳烃中，并将其作为唯一碳源[1]。虽然多环芳烃可能经历化学氧化、光分解、吸附和挥发,但在大多数情况下,多环芳烃的微生物降解是土壤中多环芳烃的主要降解方式[18,23]。尽管各种分子技术可以用来鉴定土壤中微生物群落,但我们对土壤中多环芳烃污染治理的知识仍然是有限的[6]。 (责任编辑：qin) |