亚洲大麦品种SSR指纹图谱的构建

摘要大麦是重要的粮食作物，其育种突破性进展的取得，需要种质资源的鉴定和遗传多样性分析。SSR标记作为一种特异性引物PCR分子标记，与其他分子标记相比能更好应用于大麦品种资源的遗传多样性研究。90079

本研究用CATB法提取亚洲地区不同的56份大麦的基因组DNA，利用均匀分布于DAN大麦 7 条染色体，以及筛选出的28对SSR引物对56份大麦品种进行PCR扩增，其扩增产物经2%的聚丙烯酰胺凝胶电泳，对其检测结果数据进行统计分析构建指纹图谱，根据所构建的指纹图谱对56种大麦品种进行品质鉴定和多态性遗传分析，为大麦育种提供一些理论依据。其中在28对引物中检测出多态性条带数量最多为6个，最少为 1 个，其中引物k00163，k04987，k00054，k06416 在 56 份小麦品种中检测到 1 个多态性片段，引物k09634，k03878，等检测到2个多态性片段，引物k02872，k00681，等检测到3个多态性片段，引物k04331，k05143检测到4个多态性片段，引物k0873检测到6个多态性片段，其多态性最好。多态性片段范围在100-700bp之间。

毕业论文关键词：大麦； SSR标记；指纹图谱；多态性

Abstract Barley is one of the most important food crops。 The development of its breeding progress requires the identification of germplasm resources and genetic persity analysis。Compared with other molecular markers, SSR markers can be applied to the genetic persity of Barley Germplasm with PCR markers。

In this study, the genomic DNA of 56 barley cultivars in different regions of Asia was extracted by CATB method。 56 barley cultivars were amplified by PCR using 28 pairs of SSR primers, and the amplified products were amplified by PCR。 The amplified products were analyzed by 2% polyacrylamide gel electrophoresis, and the fingerprints were analyzed by statistical analysis。Fingerprints were constructed and the quality identification and polymorphism of 56 barley cultivars were analyzed according to the constructed fingerprints, which provided some theoretical basis for barley breeding 。Among the 28 pairs of primers, the polymorphic bands were detected to be at most 6, at least one,One of the primers k00163, k04987, k00054, k06416 in 56 wheat varieties detected a polymorphic fragment,Primers k09634, k03878, etc。 detected two polymorphic fragments,Primers k02872, k00681, etc。 detected three polymorphic fragments,Primer k04331,k05143 detected four polymorphic fragments,Primer k0873 detected six polymorphic fragments, the best polymorphism。Polymorphic fragments range from 100-700 bp。

Keyword: Barley；SSR markers；fingerprints；Polymorphism

引言 1

1 材料与方法 2

1。1 材料 2

1。1。1 实验材料 2

1。1。2实验试剂及制备方法 2

1。1。3实验仪器 2

1。2实验方法 2

1。2。1样品的前处理 2

1。2。2 基因组DNA的提取 3

1。2。3 SSR-PCR反应体系 3

1。2。4 PCR扩增程序 3

1。2。5 扩增产物电泳源Q于W优E尔A论S文R网wwW.yOueRw.com 原文+QQ75201,8766 及检测 4

1。2。6 Excel软件记录数据 4

2 结果与分析亚洲大麦品种SSR指纹图谱的构建:http://www.youerw.com/shengwu/lunwen_195848.html