精胺对盐胁迫下小麦幼苗生长的影响

摘要：本实验以100mmol/L的NaCl模拟盐胁迫环境，然后通过设置三个梯度的精胺（0.05g/L,0.075g/L,0.1g/L）探究精胺缓解盐胁迫对小麦幼苗生长的影响。结果表明在盐胁迫下小麦幼苗的生长受到抑制，且SOD、POD活性升高，超氧阴离子自由基含量升高，可溶性蛋白质含量减少，叶片相对电导率升高。在加入精胺后，小麦幼苗生长的抑制作用得到了缓解，SOD、POD活性进一步升高，清除超氧阴离子自由基的能力得到增强，可溶性蛋白含量增加和叶片细胞质膜透性降低，且0.1g/L精胺对盐胁迫下小麦幼苗的缓解效果最好。

关键字：小麦，盐胁迫，精胺，缓解作用。

Abstract: In this experiment 100 mmol/L NaCl was used to simulate the environment of salt stress, and set three gradients of spermine(spm) (0.05 g/L, 0.075 g/L, and 0.1 g/L) to investigate the effects of different content of Put on the growth of wheat seedlings under salt stress.The results showed that the growth of wheat seedlings was inhibited under salt stress，and the activities of SOD and POD were increased, the content of O2•- was increased, the content of soluble protein was decreased, and the plasma membrane permeability of leaf cells was increased. After the addition of Spm, the inhibition of wheat growth was alleviated, SOD, POD activity was increased, the content of soluble protein was increased and the plasma membrane permeability of leaf cells was decreased, And 0.1g/L spermine had the best alleviation effect on wheat seedlings under salt stress.

Keywords: wheat, salt stress, spermine ,alleviation

1 前言 4

1.1盐胁迫的危害 4

1.2精胺缓解盐胁迫的作用机理 4

2 材料与方法 5

2.1试验材料 5

2.2 Hoagland营养液配制 5

2.3材料培养 5

2.4 实验设计 6

2.5 测定方法 6

2.5.1 预备、酶液的制备 6

2.5.2过氧化物酶（POD）活性的测定（愈创木酚法） 7

2.5.3超氧化物岐化酶（SOD）活性的测定（氮蓝四唑光化还原法） 7

2.5.4超氧阴离子自由基含量的测定（羟胺氧化法） 8

2.5.5可溶性蛋白含量的测定（考马斯亮蓝染色法） 8

2.5.6植物细胞质膜透性的测定（电导仪法） 9

2.6 数据分析 9

3 结果与分析 10

3.1精胺对盐胁迫下小麦幼苗株高的影响 10

3.2精胺（spm）对盐胁迫下小麦幼苗叶片POD活性的影响 11

3.3精胺（spm）对盐胁迫下小麦幼苗叶片SOD活性的影响 12

3.4精胺（spm）对盐胁迫下小麦幼苗叶片细胞质膜透性的影响 13

3.5精胺（spm）对盐胁迫下小麦幼苗叶片超氧阴离子自由基的影响 14

3.6精胺（spm）对盐胁迫下小麦幼苗叶片可溶性蛋白含量的影响 15

结论 17

致谢 19

1 前言

据联合国粮农组织（FAO）的不完全统计，全球约3419万公顷的耕地被盐渍化[1]。土壤盐渍化是现代农业生产面临的主要问题之一。随着土地利用的不断发展和大面积的增加，土壤次生盐渍化程度不断增加，土壤盐渍化面积逐年增加，盐碱地生态环境恶化，给粮食的生产带来严重威胁。引导和制约人民生活水平的提高。精胺对盐胁迫下小麦幼苗生长的影响:http://www.youerw.com/shengwu/lunwen_205143.html