黄芪黄酮提取条件优化

摘要：为确定黄芪中黄酮类成分提取的最佳工艺条件，以提取剂、乙醇浓度、固液比、提取温度、提取时间为考察因素，黄芪黄酮提取率为考察指标，采用单因素试验和正交试验优化黄芪黄酮提取工艺。本论文通过单因素实验及正交试验，获得了黄芪黄酮最佳提取工艺条件为：乙醇浓度为20 %，固液比为1:15（g/mL），提取温度为40℃，提取时间为2 h。在该提取条件下，黄芪黄酮的提取率达到172.78 mg/g，是未优化前的3.3倍。本论文的研究结果可为黄芪黄酮的提取提供技术依据。65060

关键词：黄芪，黄酮，提取，正交试验

Abstract: To determine the optimum extraction conditions of flavone in the milkvetch root, the single-factor experiment and the orthogonal experiments guided by yields of the flavone was designed. By the single-factor experiment and the orthogonal experiments, the optimum extraction conditions of flavone in the milkvetch root were obtained: the ethanol concentration was 20%, solid-liquid ratio was 1:15(g/mL), extraction temperature was 40℃, extraction time was 2h. In that extraction condition, the extraction yield was 172.78 mg/g, which was 3 times than before. The results of this paper can provide technical basis for the extraction of astragalus flavone.

Keywords: Milkvetch root, Flavone, Extract, Orthogonal experimental design

1 引言 7

1. 1 黄芪概述 7

1. 1. 1 黄芪的分类和分布 7

1. 1. 2 黄芪的化学成分 7

1. 2 黄芪黄酮提取进展 8

1. 3 本论文的研究思路 8

2 材料与方法 9

2. 1 材料与仪器 9

2. 1. 1 材料 9

2. 1. 2 药品及试剂 9

2. 1. 3 主要仪器和设备 9

2. 1. 4 试剂制备 9

2. 2 实验方法 10

2. 2. 1 提取方法 10

2. 2. 2 单因素优化黄酮的提取 10

2. 2. 3 正交设计方法 11

2. 2. 4 黄芪总黄酮含量测定方法——分光光度法 11

3 结果与讨论 13

3. 1 单因素优化结果 13

3. 1. 1 不同溶剂对黄芪黄酮提取率的影响 13

3. 1. 2 不同乙醇浓度对黄芪黄酮提取率的影响 13

3. 1. 3 不同固液比对黄芪黄酮提取率的影响 14

3. 1. 4 不同提取温度对黄芪黄酮提取率的影响 15

3. 1. 5 不同提取时间对黄芪黄酮提取率的影响 15

3. 2 正交试验优化结果 16

4 结论与展望 18

4. 1 结论 18

4. 2 展望 18

参考文献 19

致谢 20

1 引言黄芪黄酮提取条件优化:http://www.youerw.com/shuxue/lunwen_72527.html