摘要寄生蜂成虫期补充营养对寄生蜂发育适合度具有重要影响，通过补充不同糖分营养，寄生蜂可以获得更高的子代适合度。本研究通过饲喂6种不同糖分营养源，探究斑痣悬茧蜂寄生效率、成虫寿命、子代体型大小、体内营养物质累积等适合度指标的变化。结果表明，不同糖分营养对斑痣悬茧蜂寄生效率和子代体型大小无显著影响；饲喂果糖溶液的斑痣悬茧蜂成虫期寿命显著延长；成虫期全生活史饲喂不同糖分营养液后，斑痣悬茧蜂体内单糖、总糖及脂质含量显著变化，而糖原含量变化不显著。为探究寄生蜂的寄生行为对体内营养物质变化的影响，本研究首次测定了寄生行为发生前后斑痣悬茧蜂体内主要糖分和脂质的含量，结果表明，单糖、糖原及总糖含量降低，而脂质含量升高，因此，寄生行为能够显著改变寄生蜂体内营养水平。本研究取得的结果为充分探究寄生性天敌营养生理学，筛选寄生蜂保益生物防治的新途径提供了重要参考，对进一步发挥寄生性天敌的控害潜能具有重要意义。86834

毕业论文关键词：斑痣悬茧蜂；糖分营养源；寄生率；寿命；子代适合度；营养物质

Abstract The sugar supplement play an important role on the development of the parasitic wasp, and the parasitic wasps can obtain a higher degree of fitness。 The aim of present study was to determine the effect of six different sugar sources on parasitism degree, adult lifespan, offspring and accumulation of nutritional level of Meteorus pulchricornis。 The results showed that the sugar nutrition had no significant effect on parasitism efficiency and offspring body size while feeding fructose solution can prolong the adult lifespan。 The amount of monosaccharide, total sugar and lipid changed significantly while glycogen content didn’t show the same trend when feeding these six sugar solutions。 To explore the effect of parasitizing behavior on nutrient substances in the body, we conducted experiment to measure the nutritional level of adult body before and after the occurrence of parasitism behavior。 The results showed that, monosaccharide, glycogen and total sugar content decreased while lipid content increased。 Because of this, parasitic behavior can significantly change the parasitoids in nutrient levels。 Based on present study, we can fully understand the parasitoid nutrition physiology, and screen new parasitic biocontrol agent。

Keywords: Meteorus pulchricornis; sugar nutrition; parasitism rate; longevity; offspring fitness; nutrition

目  录

第一章 绪论 1

1。1 引言 1

1。2寄生蜂在生物防治中的作用 1

1。3寄生蜂的寄主调控 2

1。4寄生蜂的寄主营养利用 3

1。 5糖分对寄生蜂生长发育的影响 4

1。6花蜜补偿假说 5

1。7斑痣悬茧蜂和斜纹夜蛾寄生体系概述 6

1。8 选题的目的意义和主要研究内容 6

1。8。1 选题意义 7

1。8。2 研究目的 7

1。 8。3研究内容 8

第二章 材料和方法 9

2。1提供不同糖源物质对斑痣悬茧蜂生理的影响 9

2。2寄生蜂体内营养水平测定 10

2。2。1标准曲线制作 10