摘    要 本研究中，探究间歇性和持续性饥饿模式下，磷酸盐对长期处于饥饿条件下的厌氧氨氧化（anammox）菌的内源性代谢的影响。利用批次实验来评估anammox颗粒污泥在磷酸盐存在和不存在的情况下的不同饥饿模式的响应。在持续饥饿30 d条件下的anammox颗粒的内源性的衰减系数（基于生物量下降）为0。0062 d-1，而通过间歇饥饿，每10天提供基质可以加速anammox菌衰减，大约是持续饥饿条件下的两倍（0。0110 d-1）。然而，间歇饥饿细胞的anammox活性相对持久饥饿的细胞下降较快通过测定血红素 c（heme c）含量得出不同浓度磷酸盐对细胞生理状态的影响，低浓度的磷酸盐有利于细胞的生理状态，而高浓度的磷酸盐不利于代谢。不同的代谢策略需要依靠不同的饥饿模式，才能使anammox细菌更好的生存。通过相对较低的能源需求，以确保它们的生存条件。此外，细胞外蛋白质成分的变化表明不同的细胞内源代谢调节机制。这些结果有利于对厌氧氨氧化菌内源代谢更好的理解。90153

毕业论文关键词：厌氧氨氧化 ；饥饿；磷酸盐

Abstract Starvation of anaerobic ammonium oxidation (anammox) bacteria can occur in underloaded bioreactors or during the storage of sludge。 In this study, the impacts of intermittent and persistent resting modes and phosphates on the endogenous metabolism of anammox bacteria during long-term starvation were investigated。 Batch experiments were performed to evaluate the response of anammox granules to different modes of starvation (persistent-resting and intermittent-resting cells) in the presence and absence of phosphates。 The endogenous decay coefficient of anammox granules (based on the decreased biomass) over a 30-d persistent starvation period was 0。0062 d-1, while the intermittent starvation by means of providing substrates every 10 days accelerated the biomass decay approximately twice (0。0110 d-1)。Moreover, the intermittent-resting cells displayed exacerbated decreases in specific anammox activity relative to the persistent-resting cells, whereas no significant difference was observed in the heme c contents。 Regardless of the physiological status of the cells, the presence of low levels of phosphates may be beneficial; however, a high level of phosphates is not recommended。 Although different metabolic strategies were adopted depending on the starvation mode, anammox bacteria possessed a better survival strategy via a relatively low maintenance energy requirement to ensure their survival in nutrient-limited systems。 In addition, the variations in extracellular protein-like components were indicative of different intracellular metabolic regulation mechanisms。 These results provide a better understanding of the endogenous metabolism of anammox bacteria。

Keyword:  anammox; malsato; fosfato

目录

引言 5

1。 材料和方法 5

1。1污泥的源Q于W优E尔A论S文R网wwW.yOueRw.com 原文+QQ75201,8766 来源 5

1。2饥饿批次实验 5

1。3血红素提取和测定 6

1。4 EPS提取 6

1。5生物衰减速率的测定 6

1。6分析方法 8

1。7统计分析 8

2．结果与讨论 8

2。1生物衰减速率 8

2。2生理动力学 9

2。3磷酸盐的影响 10

2。4 EPS数量和组成的变化