CaSR介导色氨酸诱导猪胃胃肠激素分泌的规律
毕业论文关键词：猪；色氨酸；钙敏感受体；胃肠激素；灌流
Law of CaSR - mediated tryptophan - induced secretion of gastrointestinal hormones in porcine
Abstract:[Objectives]The effects of tryptophan on the secretion of gastrointestinal hormones in gastrointestinal tract of pigs were studied by measuring the secretion of gastrin and somatostatin in the perfusion experiments under different conditions.[Methods]Three of the different treatments were used to perform the perfusion experiments. The variables of experiment 1 were the concentration of tryptophan in perfusate. The variables of experiment 2 were CaSR activators. The variables of experiment 3 were tryptophan and CaSR Inhibitors.[Results]After the addition of tryptophan, the gastrointestinal hormone concentration was significantly different from that of the control group(P < 0.05). When the CaSR activator was added, the gastrointestinal hormone concentration was significantly different from that of the control group(P < 0.05). When the CaSR inhibitor was added, the concentration of gastrointestinal hormones was not significantly different from that of the control group(P > 0.05).[Conclusions]Tryptophan can promote gastrointestinal hormone somatostatin, gastrin secretion activity, this pro-secreted effect through CaSR mediated implementation.
Key words: Pig；Tryptophan；CaSR；gut hormone；perfusion
目 录
摘要1
关键词1
Abstract1
Keywords1
引言1
1材料与方法2
1.1建立表面灌流系统2
1.2试剂和仪器2
1.3样品处理3
1.3.1配置Kreb’s培养液3
1.3.2组织处理3
1.4试验设计与样品收集3
1.4.1灌流实验3
1.4.2试验处理3
1.5指标测定方法3
1.6数据统计分析3
2结果与分析3
2.1 Trp对猪胃生长抑素、胃泌素分泌活性的影响3
2.2 CaSR激活剂处理猪胃生长抑素、胃泌素分泌活性的影响4
2.3 CaSR抑制剂处理对Trp诱导生长抑素、胃泌素分泌活性的影响4
3讨论5
4结论5
致谢6
参考文献6
CaSR介导色氨酸诱导猪胃胃肠激素分泌的规律
胃肠道除除具有消化吸收功能外，也执行着重要的内分泌功能。胃肠道内分泌细胞有A、B、D、H、G、I、K、L、N、P、S、EC等[1]，这些细胞在受到某些物质的刺激之后，可以分泌出许多代谢相关的激素，统称为胃肠激素。其中常见的有生长抑素（SS，由D细胞分泌）、胰高血糖素样肽（GLPs，由L细胞分泌）、胆囊收缩素(CCK，由I细胞分泌)、胃泌素(gastrin，G细胞分泌)等。
胃泌素(gastrin)是人类最先发现的激素之一，1905年首先被伦敦大学的Edlkins发现并命名，后来证明其属于多肽，是胃泌素族的重要成员[2]。胃泌素主要是由胃窦和小肠的上部粘膜G细胞释放。刺激胃酸分泌是胃泌素的主要功能。生长抑素(SS)是一种抑制脑垂体分泌生长激素的神经调节肽，广泛分布于脊椎动物的中枢和外周神经系统、胃肠道等组织，SS 的主要功能包括抑制细胞增殖、神经递质释放、抑制胃酸、胃泌素及胃蛋白酶的分泌和刺激黏液分泌等[3]。