大丽轮枝菌侵染不同棉花品种激发棉花的防御反应研究
关键词：棉花；黄萎病；大丽轮枝菌；防御反应
Research Advances in the Interaction Mechanism Between Cotton and Verticillium dahliae
Abstract： Cotton Verticillium has become one of the critical diseases to restrict the cotton production, which lead to serious loss in yield and fiber quality. Moreover, there isn’t an efficient way to control the disease. This disease is a soil-borne and seed-borne vascular disease, the main causative pathogen is Verticillium albo-atrum and Verticillium dahlia. While the majority pathogenic bacteria is Verticillium dahliae in Chinese cotton-producing areas. After the infection by Verticillium dahlia, the tissue structure of cotton will response with a series of changes to resist the invasion of pathogens, and in process of the interection with pathogens the cotton will produce a series of defense reactions to resist further invasion of pathogens. This study adopted the Verticillium dahliae which marked by green fluorescent protein (GFP) to effect six different varieties of cotton, study on the responses when sdifferent resistance cotton varieties was infected by Verticillium dahlia. Research showed that the immune response was related to the resistance enhancement after Verticillium dahliae infection, different varieties of cotton were inoculated with Verticillium dahliae spore suspension, the resistances of cultivars were evaluated. The Verticillium dahliae biomass indifferent parts of cotton were negatively related with resistance level, and lignin content of hypocotyl, leaves callose deposition and hydrogen peroxide accumulation increased with the enhancement of disease resistance, the defensive enzymes activity like polyphenol oxidase (PPO), peroxidase (POD) and catalase (CAT) were positively related with disease resistance.
Key words: Cotton; Verticillum wilt; Verticillium dahlia; Defense response
摘要1
关键词1
Abstract1
Key words1
引言（或绪论）2
1 材料与方法2
1.1 实验材料 2
1.1.1供试材料与菌株2
1.1.2实验仪器2
1.1.3实验试剂2
1.2 实验方法 2
1.2.1培养棉苗2
1.2.2大丽轮枝菌孢子悬浮液制备2
1.2.3不同棉花品种的黄萎病抗性鉴定3
1.2.4大丽轮枝菌侵染的显微观察以及生物量检测3
1.2.5木质素测定3
1.2.6胼胝质检测4
1.2.7过氧化氢（H2O2）测定4
1.2.8酶活性测定4
1.2.9数据分析4
2 结果与分析5
2.1不同棉花品种的黄萎病的抗性5
2.2 植株中大丽轮枝菌的生物量5
2.3大丽轮枝菌对棉花根部木质素含量的影响6
2.4大丽轮枝菌处理后棉株叶片的胼胝质沉积6
2.5叶片过氧化氢积累7