茶树叶发育相关的CsGIF1基因克隆与功能鉴定
毕业论文关键词：茶树；CsGIF1；叶发育；激素刺激
Cloning and functional identification of leaf development-related CsGIF1 genes in Camellia sinensis
Abstract： Tea plant (Camellia sinensis (L.) O. Kuntze) is an important leaf-type woody crop used for producing of natural non-alcoholic beverages. The yield of tea plant leaf is closely related to the economic benefit. GRF-INTERACTING FACTOR (GIF) genes play an important role in regulating the process of leaf expansion. It can positively regulate the leaf development cooperating with GROWTH-INTERACTING FACTOR (GRF). In the study, CsGIF1 in the leaf of C. sinensis was cloned and identified. The results showed that length of CsGIF1 were 666 bp, encoding 221 amino acids, and it has a highly conserved region called SNH domain. Protein analysis suggested that the CsGIF1 was hydrophily protein, and its theoretical relative molecular weight is 23,380, theoretical isoelectric point is 6.30, percentage of acidic, basic, aromatic and aliphatic amino acids is 7%, 9%, 5% and 13%. Secondary structure prediction showed that CsGIF1 protein consists of 38.01% alpha-helix, 10.41% beta turn, 12.22% extended strand and 39.37% random coil. The expression profiles of CsGIF1 gene were higher in tender leaves and consistently downregulated during tea leaf development. Those results suggested that CsGIF1 may be actively involved in the early stage of leaf tissue formation in tea plant. The pergence of CsGIF1 in response to different hormonal stimuli revealed the possible multiple functions of these genes in hormonal regulation. This study provided the molecular basis of the CsGIF1 gene for future functional research on leaf development and hormonal stimuli in C. sinensis.
Key words: Camellia sinensis；CsGIF1；Leaf development；Hormonal stimuli
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1 材料与方法2
1.1 材料及处理 2
1.2 方法 3
1.2.1 总RNA的提取及cDNA的合成3
1.2.2 茶树CsGIF1基因的克隆3
1.2.3 序列分析4
1.2.4 定时定量PCR4
2 结果与分析4
2.1茶树CsGIF1基因的克隆4
2.2茶树CsGIF1基因编码氨基酸序列与不同物种的GIF蛋白氨基酸序列同源性比较分析5
2.3 系统进化分析6
2.4茶树CsGIF1预测蛋白序列同其他物种的蛋白质理化特性分析6
2.5茶树CsGIF1预测蛋白氨基酸序列亲水性/疏水性分析7
2.6 茶树CsGIF1转录因子的二级结构预测与分析8
2.7茶树叶片发育过程中CsGIF1基因的表达8
2.8不同植物激素处理下CsGIF1基因的表达8
3 讨论9
致谢10
参考文献10
图1 不同发育阶段茶树叶片3
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