两广唇柱苣苔的扦插繁殖和半致死温度研究
毕业论文关键词： 两广唇柱苣苔；扦插繁殖；半致死温度；电解质渗出率；叶绿素荧光
Cutting propagation and Semi-lethal temperature of Chirita sinensis
Abstract： Studing cutting propagation and semi-lethal temperature of Chirita sinensis by contrast experiment,the result shows that the efficiency of cutting survival rate of Chirita sinensis is high,and growth hormone have obvious influence on it.By contras,Auxin NAA is more effective than Auxin IBA.Comparing different leaf cutting ways to propagate proves that two-stages leaf cutting is efficient.Studing emi-lethal temperature of Chirita sinensis proves that the electrolyte permeability rapidly increased at sudden loading operation when temperature is above 40℃ to 45℃ and drops to 5℃-0℃,the critical temperature can be determined.Membrane is hurted in those temperature range and it makes permeability increased,as investigation of Ions is enhanced,plant have transport mechanisms response.Electrolyte permeability presentes the "S" type curve as the temperature increases, it has high degree of fitting between electrolyte permeability and Logistic equation.According to the calculation,semi-lethal temperature of Chirita sinensis is 42.5℃ and 3.34℃.
Keywords： Chirita sinensis; Cutting propagation ; semilethal temperature; electrolyte permeability ;Chlorophyll Fluorescence
目录
1 绪论 3
1.1苦苣苔科植物概述 3
1.1.1 苦苣苔科植物的分类及分布情况 3
1.1.2 苦苣苔科植物的形态特征及生态习性 3
1.1.3 苦苣苔科植物的应用及研究现况 3
1.2两广唇柱苣苔概述 3
1.2.1 两广唇柱苣苔形态特征及生态习性 4
1.2.2 两广唇柱苣苔的栽培要点及繁殖方法 4
1.3 半致死温度 5
1.3.1 应用logistic方程测定植物半致死温度的原理 5
1.3.2 应用logistic方程测定植物半致死温度的拟合方法 5
1.3.3 应用logistic方程测定植物两广唇柱苣苔半致死温度的意义 6
1.4 叶绿素荧光 6
1.4.1 测定两广唇柱苣苔叶绿素荧光的意义 6
2 材料与方法 8
2.1 实验材料 8
2.2 实验方法 8
2.2.1 不同叶片切割方式 8
2.2.2 植物生长调节剂处理 9
2.2.3 两广唇柱苣苔半致死温度测定的实验方法 9
3 结果与分析 11
3.1 两广唇柱苣苔扦插繁殖实验 11
3.2 半致死温度测定 12