水稻转录因子OsMADS57对水稻氮素积累的影响
毕业论文关键词：水稻；OsMADS57；转录因子；氮素积累
Effects of Rice Transcription Factor OsMADS57 on Nitrogen Accumulation in Rice
Abstract：NO3- plays an important role not only in promoting plant growth and development, but also as a signal to regulate plant growth and development. The OsMADS57 mutant and overexpression of different nitrogen concentration and nitrogen conditions revealed that, compared with the wild type, OsMADS57 mutant root 0.2 mM NO3- under the conditions of shorter, lower biomass, and rice in 5 mM NO3-and 0.2 mM NH4+ under the condition of growth was not affected. Through the determination of nitrogen concentration, 15N and SAP nitrate found that, compared with the wild type, 0.2 mM under the condition of NO3- osmads57 mutant of nitrate nitrogen translocation from roots to shoots was inhibited, while the nitrate uptake did not change, 0.2 mM under the treatment of NO3- OsMADS57 over expression transgenic root NO3- to increase the ground transport. Found that the transporter gene expression detection under 0.2 mM NO3-, compared with the wild type, the down-regulation of OsNRT2.1/2.2/2.3a/2.4 expression in the OsMADS57 mutant, OsMADS57 expression OsNRT2.1/2.2/2.3a/2.4, OsNAR2.1 and OsNia1/2 were significantly increased.
Key words:Rice;OsMADS57;Transcription factor;Nitrogen Accumulation
目 录
摘要3
关键词3
Abstract3
Key words3
1引言3
2材料方法4
2.1实验材料 4
2.2OsMADS57突变体鉴定 4
2.2.1osmads57T-DNA插入突变体的鉴定4
2.2.2 CRISP-CAS9介导突变体的获得5
2.3OsMADS57超表达材料的创建5
2.3.1 OsMADS57超表达载体的构建5
2.3.2 OsMADS57超表达材料的鉴定 6
2.4突变体和超表达植株表型及氮素含量分析 7
2.4.1 突变体和超表达不同氮浓度条件下对水稻植株生长和氮素积累的影响 7
2.4.2 突变体和超表达植株不同氮浓度条件下15N的吸收和转运 7
2.4.3突变体和超表达植株不同氮浓度条件下木质部伤流液的收集7
2.4.4 突变体和超表达植株N浓度的测定7
2.4.4.1突变体植株NO3-N浓度的测定7
2.4.4.2 突变体植株全氮浓度的测定8
2.5突变体和超表达植株相关基因表达分析8
2.6数据处理8
3结果与分析9
3.1.突变体和超表达材料的获得及分子鉴定9
3.1.1.突变体材料的获得和分子鉴定9
3.1.2.OsMADS57超表达材料的分子鉴定10
3.2.突变体和超表达材料的表型分析及氮素积累11
3.2.1.突变体不同硝态氮浓度条件下的表型分析及氮素积累11
3.2.2 突变体不同N形态条件下的表型分析及氮素积累13
3.2.3.OsMADS57超表达材料不同硝态氮浓度条件下的表型分析及氮素积累14
3.3.OsMADS57沉默突变体和超表达植株15N的吸收与转运16
3.3.1.OsMADS57沉默和超表达材料的15N吸收16
3.3.2.OsMADS57沉默和超表达材料15N的积累16
3.4.OsMADS57沉默突变体和超表达植株伤流液中硝态氮的转运量17