葡萄籽中白藜芦醇的提取及初步纯化
毕业论文关键词：白藜芦醇；葡萄籽；复合酶解法；白藜芦醇得率；双水相；分配系数
Abstract: This paper adopted complex enzymatic hydrolysis to extract resveratrol from Xinjiang Turpan grape seed, and studied the distribution of behavior in ammonium sulfate and polyethylene glycol aqueous two-phase system. Choosing different enzyme dosage, enzyme ratio, reaction temperature, solid-liquid ratio to get on the single factor test ,then using L¬9（34） for enzymatic extraction process conditions resveratrol orthogonal test to extract resveratrol technology.The optimized extraction conditions were as follows:composite enzyme amount, 2.0 ml;compound enzyme ratio,1:1;temperature,50 ℃; material liquid ,1:16 (g/ml), the resveratrol extraction rate is 0.94%. On the basis of digestion on the use of two water distribution behavior of resveratrol in two phases phase detection, the choice of PEG / (NH4) 2SO4 aqueous two-phase systems to be tested.The optimized extraction conditions were as follows: molecular weight of PEG ,1000; mass fraction of PEG,25%; mass fraction of (NH4)2SO4 ,45% ;pH, 8.0.
Key Words: Resveratrol; grape seed; composite enzymatic hydrolysis; yield; aqueous two-phase system; partition coefficient
目 录
1 前言 1
1.1 葡萄籽粉简述 1
1.2 白藜芦醇简述 1
1.3 白藜芦醇的生物活性 2
1.3.1 抗癌活性 2
1.3.2 对心血管系统的作用 2
1.3.3 激素样作用 3
1.3.4 其他作用 3
1.4 白藜芦醇的提取 3
1.5 白藜芦醇的前景 4
1.6 研究目的和意义 5
2 材料与方法 6
2.1 实验材料与试剂 6
2.2 实验仪器 7
2.3 实验方法 7
2.3.1 标准曲线的制作 7
2.3.2 复合酶解法单因素 8
2.3.3 复合酶解法提取工艺的优化 9
2.3.4 两水相体系对白藜芦醇分配行为的影响 9
3 实验结果与讨论 11
3.1 白藜芦醇标准曲线 11
3.2 复合酶解法单因素试验 12
3.2.1 复合酶添加量对白藜芦醇得率的影响 12
3.2.2 温度对白藜芦醇得率的影响 13
3.2.3 复合酶比例对白藜芦醇得率的影响 14
3.2.4 料液比对白藜芦醇得率的影响 15
3.2.5 正交试验工艺优化 16
3.3 两水相体系对白藜芦醇分配行为的影响 16
3.3.1双水相相图的制作 16
3.3.2 PEG分子量对白藜芦醇浓度的影响 18
3.3.3 PEG质量分数对白藜芦醇分配系数的影响 19