Microbulbifersp.A4B-17菌株的对羟基苯甲酸合成研究
毕业论文关键词：对羟基苯甲酸；高效液相色谱；生物合成
Study on the synthesis of 4-hydroxybenzoic acid by Microbulbifer strain sp.A4B-17
Abstract: Microbulbifer sp. A4B-17 strain with synthesis of p-hydroxybenzoic acid (4-Hydroxybenzoic, acid, 4HBA) and the ester function.4HBA in industrial production and daily life has a lot of important role, such as in industrial dye for synthesis of heat sensitive dye chromogenic agent; can be used for color film and synthetic oil soluble couplers 538 and nylon 12 as plasticizing agent in the production of raw materials; also can be used for liquid crystal polymers and plastics.This study designed to explore the best carbon source for the A4B-17 strain synthesis 4HBA and its esters, for A4B-18 strains developed into efficient industrial strains to lay the foundation, so as to make the production process more clean and environmentally friendly.The experimental results are as follows: (1) in seawater inorganic training base (ONR7a) were added sugars as sole carbon source of A4B-17 were cultured, found in addition to pyruvate other carbon sources of growth are not very good, may because of the strain required growth factor.(2) in order to make a quantitative analysis on the 4HBA prepared different concentrations of 4HBA performed by high performance liquid chromatography analysis draw the standard curve, then A4B-17 strains in 4 kinds of different culture media culture, and analyses on the synthesis of 4HBA quantitative. The results show that the MB medium, seawater nutrient medium, glucose culture medium and pyruvate culture medium were synthesized0.057mMol/L,0.007mMol/L, and 0.00059mMol/L 0.00068mMol/L 4HBA.
Key words:4-hydroxybenzoic acid;High efficiency liquid chromatography;Biosynthesis
目录
引言 1
1 材料与方法 2
1.1供试材料 2
1.1.1菌株和预制4HBA 2
1.1.2 试剂 2
1.1.3培养基 2
1.2实验仪器 3
1.3实验方法 4
1.3.1 唯一碳源的利用 4
1.3.2高效液相色谱分析 5
2 实验结果及分析 6
2.1唯一碳源的利用 6
2.1.1代谢途径的分析 7
2.1.2碳源的利用情况 8
2.2高效液相色谱分析 9
2.2.1对羟基苯甲酸以其酯类的标准曲线绘制 9
2.2.2不同碳源培养后高效液相色谱分析 11