摘要体外模型实验研究了芹菜素对 α-葡萄糖苷酶的抑制活性，结果表明，芹菜素的抑 制活性呈浓度效应，芹菜素浓度为 25 μg/mL 时，抑制率为 45。7%，IC50 值为 26。3 μg/mL， 与阳性对照阿卡波糖的抑制率相当。考察不同金属离子对 α-葡萄糖苷酶的抑制活性。 结果表明，Cu2+、Zn2+、Mn2+、Mg2+、Ca2+、Fe3+对 α-葡萄糖苷酶均具有较好的抑制 作用，IC50 值分别为 92。8 μmol/L、107。4 μmol/L、273。2 μmol/L、422 μmol/L、327。2 μmol/L、216。4 μmol/L。进一步考察上述金属离子与芹菜素的协同抑制活性。结果显 示：芹菜素浓度为 1 μg/mL 时，随着 Cu2+浓度的增加，抑制率先上升后下降，Cu2+浓 度为 140 μmol/L，抑制率为 69。6%。芹菜素浓度为 4 μg/mL 时，随着 Zn2+浓度的增加， 抑制率先上升后下降，Zn2+浓度为 70 μmol/L，抑制率为 51%。芹菜素浓度为 2。5 μg/mL 时，随着 Fe3+浓度的增加，抑制率呈线性上升，Fe3+的浓度为 33 μmol/L，抑制率为 55。5%。芹菜素浓度为 15 μg/mL 时，随着 Mn2+浓度的增加，抑制率呈线性上升，Mn2+ 浓度为 125 μmol/L，抑制率为 40。4%。芹菜素浓度为 0。9 μg/mL 时，随着 Ca2+浓度的 增加，抑制率呈线性上升，Ca2+浓度为 35 μmol/L，抑制率为 51。4%。芹菜素浓度 2。5 μg/mL 时，随着 Mg2+浓度的增加，抑制率先上升后下降，Mg2+浓度为 80 μmol/L，抑 制率为 52。6%。80970

毕业论文关键词：芹菜素；α-葡萄糖苷酶；金属离子；协同抑制

Abstract The inhibition activities of apigenin against alpha-glucosidase were assayed, the results displayed that the inhibition activities were increased with the incresing of apigenin concentrations, the inhibition rate was 45。7% when apigenin concentration was 25 μg/mL, and IC50 was 26。3 μmol/L, which was similar with the inhibition activities of acarbose。 The inhibition activities of different metal-ions against alpha-glucosidase were assayed, the results showed that Cu2+,Zn2+,Mn2+,Mg2+,Ca2+,Fe3+had effective inhibition on the alpha- glucosidase, IC50 was 92。8 μmol/L, 107。4 μmol/L, 273。2 μmol/L, 327。2 μmol/L, 422 μmol/L,

216。4 μmol/L。Further,synergetic inhibition of different metal-ions and apigenin on alpha- glucosidase was assayed, the results showed that when apigenin concentration was 1 μg/mL, with the increase of Cu2+concentration, inhibitory rate rwase first and reduced latter, the inhibitory rate was 69。6%, when Cu2+ concentration was 140 μmol/L。 When apigenin concentration was 4 μg/mL, with the increase of Zn2+concentration, inhibitory rate rwase first and down latter,the inhibitory rate was 51%, when Zn2+concentration was 70 μmol/L。 When apigenin concentration was 2。5 μg/mL, with the increase of Fe3+ concentration,the inhibitory rate rwase linearly, the inhibitory rate was 55。5%, when the Fe3+ concentration was 33 μmol/L。 When the apigenin concentration was 15 μg/mL, with the increase of Mn2+concentration, the inhibitory rate rwase linearly, the inhibitory rate was 40。4%,when Mn2+ concentration was 125 μmol/L。 When apigenin concentration was 0。9 μg/mL, with the increase of Ca2+ concentration,the inhibitory rate rwase linearly, the inhibitory rate was 51。4%。, when Ca2+ concentration was 35 μmol/L。 When apigenin concentration was 2。5 μg/mL, with the increase of Mg2+ concentration,the inhibitory rate rwase first and down latter, the inhibitory rate was 52。6%, when Mg2+ concentration was 80 μmol/L。

Key words: apigenin; alpha-glucosidase; meta-lion; synergwastic inhibitory

目 录

第一章 绪论 1

1。1 前言 1

1。2