摘要：芒毛苣苔属为苦苣苔科多年生草本植物，具有较高的观赏价值和药用价值，但缺少对其人工栽培及其相关生理的研究报道。本试验通过以不同温度的高温和低温处理芒毛苣苔叶圆片，测定叶片溶液电导率变化，即电解质渗出率的变化，利用logistic方程拟合，并计算它们的高温和低温的半致死温度 (LT50)。结果表明，九种芒毛苣苔属植物叶片高温或低温处理后电解质渗出率均呈现“S”型曲线变化，利用Logistic方程拟合并计算得出这九种芒毛苣苔的高温和低温半致死温度，各种类的高温半致死温度差别较小（最低39。2℃，最高44。5℃），而各种类低温半致死温度差别较大，其中束花芒毛苣苔（Aeschynanthus hookeri）拥有较高耐寒性，半致死温度-13℃。勐醒芒毛苣苔（Aeschynanthus mengxingensis）耐寒性较弱，半致死温度11。7℃。这可以为该九种芒毛苣苔属植物的栽培温度条件提供参考依据。81888

毕业论文关键词：芒毛苣苔；半致死温度；相对电导率；Logistic方程

Semi-lethal temperature of several Aeschynanthus species and adaptability research

Abstract:  Aeschynanthus are perennial herb of Gesneriaceae genus, with high ornamental value and medicinal value, but lack of research reports on the artificial cultivation and physiology。 This experiment used different temperature to treatment Aeschynanthus acuminatus leaf discs, measuring the changes of electrolyte leakage rate, use logistic equation to calculate the semi-lethal temperature(LT50) of high and low temperature。The results show that the treatment with the temperature increases or decreases, electrical conductivity of nine kinds of Aeschynanthus acuminatus showed "S" type change, through the significant test, accord with the Logistic equation。 And obtaine the data of semi-lethal temperature of nine kinds of Aeschynanthus acuminatus。The high temperature semi lethal temperature of each kind of difference is small (minimum 39。2℃, highest 44。5℃)。 While various semilethal temperature differences, Aeschynanthus hookeri showed high cold resistance, semi lethal temperature -13℃。 Aeschynanthus mengxingensis cold resistance is weak, the semi lethal temperature is 11。7℃。This can provide a physiological basis for cultivation to Aeschynanthus acuminatus。

Key words: Aeschynanthus; semi-lethal temperature; relative electrical conductivity; Logistic equation 

目  录

1 绪论 1

1。1 苦苣苔科植物 1

1。2 芒毛苣苔属植物的形态特征和分类 1

1。3 芒毛苣苔属植物的园艺应用 2

1。4 芒毛苣苔属植物的半致死温度以及研究意义 3

2 材料与方法 4

2。1 试验材料的处理 4

2。2 试验的时间及地点 7

2。3 试验主要应用仪器和设备 7

2。2 试验内容 7

2。4。1 高温胁迫下芒毛苣苔半致死温度测定 7

2。4。2 低温胁迫下芒毛苣苔半致死温度测定 7

2。4。3  Logistic方程拟合和半致死温度计算 7

3 结果与分析 8

3。1总体分析 8

3。1。1 高温半致死温度 8

3。1。2 低温半致死温度 8