摘要：本课题以罗汉果为主要研究对象。首先，通过单因素实验和正交试验来确定罗汉果甜甙的最佳提取工艺，运用紫外分光光度法对提取出的罗汉果甜甙的含量进行测定。然后将提取出的甜甙加至卷烟中，并进行人工感官评价；再通过电子舌对卷烟样品进行主成分分析（PCA）、判别因子分析（DFA）和聚类分析（CA），构建觉指纹图谱比较不同甜甙浓度的卷烟样品间觉的差异性；通过GC-MS分析卷烟烟气。实验结果表明：最终确定浸提温度30℃、浸提时间60min、投物料液比1:30为最佳反应条件；不同甜甙浓度的卷烟样品间的感官属性具有显著差异；电子舌可以清晰地区分不同的卷烟样品；4种卷烟样品共检测出160种香气成分，其中醇类、酮类、芳香族以及杂环类化合物是主要挥发性成分。57619

毕业论文关键字：罗汉果；甜提取物；紫外分光光度法；卷烟烟气；超声提取技术

The application of the sweet extracts from Momordica Grosvenori to the cigarettes

Abstract: Momordica grosvenori is the main objects of study. Firstly, according to single factor test and the orthogonal test, the optimum processing of extracting mogrosides was built and measuring the content of mogrosides by ultraviolet spectroscopy. Then the mogrosides was added to the cigarettes and the sensory evaluation was carried on the cigarettes. The Principal Component Analysis (PCA), Discriminant Factorial Analysis (DFA), Cluster Analysis (CA)  and building the taste fingerprints of the cigarettes with different concentrations of mogrosides were used to compare the difference on the taste of the samples by electronic tongue. The cigarette smoke was analyzed by GC-MS tests. The results indicated extraction temperature for 30℃, extracting time for 60 min and solid to liquid for 1:25 were best synthetical condition which was decided. The cigarettes with different concentrations of mogrosides sensory attributes have significant difference. The electronic tongue can clearly distinguish five kinds of the cigarettes samples. Four kinds of different cigarettes samples were detected in 160 kinds of aroma components, including alcohols, ketones, aromatic compounds and heterocyclic compounds were the main aroma components.

Key Words: Momordica grosvenori; the sweet extracts; Ultraviolet spectroscopy; smoke from

 cigarettes; ultrasonic extraction technics 

目  录

1 引言 1

1.1罗汉果的甜成分 1

1.2甜成分的提取 1

1.2.1超声波提取的原理 2

1.2.2超声波辅助技术的特点 2

1.2.3超声波辅助提取的影响因素 3

1.3甜成分的检测 4

1.4卷烟烟气的形成 5

1.5卷烟烟气的分析方法 5

1.6卷烟觉特征分析 6

1.6.1感官评定 6

1.6.2电子舌 7

1.7 本课题研究目的及意义 7

1.8 本课题研究的主要内容 8

2 实验材料和方法 9

2.1 实验材料 9

2.1.1实验材料、试剂及样品信息 9

2.1.2 主要仪器及设备 10

2.2 实验方法 10

2.2.1紫外分光光度法