摘要：本课题以葛根为原料，利用超声波提取其中的黄酮类物质，并用双水相萃取体系对它进行初步的分离纯化。利用单因素实验和正交实验对超声提取的工艺条件进行优化；通过对比四种双水相体系的分相能力和萃取能力，确定最适的双水相萃取体系，并对萃取体系的最佳纯化条件进行了研究。结果表明：超声辅助提取葛根黄酮类物质的最佳条件为：超声时间30min，超声功率为150W，超声温度为60℃，料液比为1:20。最佳萃取体系为乙醇/磷酸氢二钾双水相体系，该体系得到最佳萃取条件：乙醇质量分数为42.86%、磷酸氢二钾质量分数为26.42%，pH为8。61940

毕业论文关键词：葛根；黄酮；超声提取；双水相萃取

Study on Ultrasonic Assisted Extraction of Flavonoids from Puerarin and Preliminary Purification

Abstract: In this paper Puerarin as raw material, the use of ultrasonic extract of flavonoids and aqueous two-phase extraction system with its preliminary separation and purification. By single factor and orthogonal experiments on ultrasonic extraction process conditions are optimized; the comparison of four kinds of aqueous two-phase system of phase separation and extraction ability to determine the optimal aqueous two-phase extraction system and the best extraction system purification conditions. The results showed that ultrasonic assisted extraction of flavonoids in Pueraria optimal conditions is ultrasonic time of 30min, ultrasonic power of 150W, ultrasonic temperature of 60 ℃, solid-liquid ratio of 1:20. The best system for the extraction is ethanol / dibasic potassium phosphate aqueous two-phase system which is optimum extraction conditions: ethanol mass fraction of 42.86%, dipotassium hydrogen mass fraction of 26.42%, pH 8.

Keywords: puerarin; flavonoids; ultrasonic extraction; aqueous two-phase extraction

目录

1绪论 1

1.1 葛根黄酮介绍 1

1.2 黄酮类物质提取方法 2

1.2.1 微波提取法 2

1.2.2 超临界流体萃取 2

1.2.3 超声波提取 3

1.2.4 双水相萃取技术 3

1.3 目的与研究意义 5

2材料与方法 6

2.1 材料、试剂及其配制方法 6

2.2 仪器设备 6

2.3 葛根黄酮含量测定 6

2.3.1 葛根素标准曲线 6

2.3.2 总黄酮的测定 7

2.4 超声辅助提取葛根黄酮的工艺条件优化 7

2.4.1 超声时间对提取率的影响 7

2.4.2 超声功率对提取率的影响 8

2.4.3 超声温度对提取率的影响 8

2.4.4 料液比对提取率的影响 8

2.4.5 乙醇浓度对提取率的影响 8

2.4.6 黄酮类物质提取正交实验优化 8

2.5 供试液（葛根醇提浓缩液）的制备 9

2.6 双水相分离纯化供试液 9

2.6.1 双水相体系分相能力的研究 9

2.6.2 双水相体系萃取能力的测定 9

2.6.3 乙醇-磷酸氢二钾双水相体系纯化条件优化