摘要：青霉素滥用所造成的环境水样残留问题日益受到关注。本论文使用的是一种高选择性、高灵敏度快速检测水样中青霉素残留的方法。表面增强拉曼光谱(SERS)技术具有灵敏度高，选择性好，水的干扰小，样品用量少等优点。本实验利用电沉积法制备了一种基于纳米金-石墨烯的青霉素分子印迹传感器，用表面增强拉曼光谱对其进行表征，将其应用于水样中青霉素残留的SERS检测。干扰实验表明四环素对青霉素的检测没有干扰。本实验检测了pH的最优范围是pH=7.0。同时，随着青霉素的浓度的增加，SERS的峰也随之增高。印迹材料检出限为8.0*10-8mol/L。71799

毕业论文关键字: 青霉素;表面增强拉曼光谱;分子印迹

Preparation of Molecular Imprinting SERS Sensor and Its Application in Penicillin Monitoring

Abstract：The problem of environmental water sample residue caused by the abuse of penicillin has received increasing attention. The thesis used a highly selective and highly sensitive method for rapid detection of penicillin residues in water samples. The advantages of surface enhanced Raman spectroscopy (SERS) are high sensitivity, good selectivity, little water disturbance and less sample size. In this experiment, the molecular imprinting sensor based on grapheneaned gold nanoparticles was prepared by electrodeposition method, and characterized by surface enhance Raman spectroscopy .It was applied to SERS detection of penicillin residues in water samples. Interference experiments showed that tetracycline did not interfere with the detection of penicillin. The optimal range of pH for this experiment was pH = 7.0. At the same time, the peak of SERS increased with the increase of the concentration of penicillin. The detection limit of imprinted material was 8.0* 10-8mol / L.

key words: penicillin; SERS; Molecularly Imprinted

目录

1  绪论 5

1.1 青霉素 6

1.1.1 简介 6

1.1.2青霉素的发现 6

1.1.3 青霉素的种类 6

1.1.4抗生素污染 6

（1）微生物抑制检测法 7

（2） 快速检测试剂盒 7

（3）免疫法 7

（4）理化检验法 8

（5）生物传感器检测法 8

1.2 表面增强拉曼散射原理与纳米材料 8

1.2.1  SERS基底的制备 9

（1）纳米银 10

（2）Fe3O4磁性纳米粒子 10

（3）碳纳米颗粒 10

（5） 石墨烯 11

（6） 纳米金 11

1.3 分子印迹 12

1.3.1分子印迹在SERS中的应用 12

1.3.2 分子印迹的常用制备方法 13

（1）本体聚合 13

（2）分散聚合与沉淀聚合 13

（3）悬浮聚合 13

（4）表面分子印迹 13

（5）原位聚合 13

（6）抗原印迹技术 13

1.4本课题的研究背景与原理