摘要：表面增强拉曼散射技术具有灵敏度高、检出限低、速度快等特点，其被广泛应用于生物检测、环境监测、食品安全和药物分析等领域。本文对表面增强拉曼活性基底进行了表面改性，并利用表面增强拉曼散射技术对农药啶虫脒进行了定量检测，为农药残留提供了一种新的检测方法和技术手段。首先，利用稀释的硝酸对具有表面增强活性的新型银纳米阵列基板进行了表面改性，并采用激光波长为785 nm 的拉曼光谱仪对纳米探针分子 1,2-二(4-吡啶基)乙烯(BPE)进行了检测，获得了不同浓度硝酸清洗下的表面增强拉曼光谱。结果表明，当硝酸浓度为4.914 M 时， BPE 的检测限可达到10-8 M，其灵敏度比表面改性之前至少提高了 100倍。其次，对不同浓度的农药啶虫脒表面增强拉曼光谱进行了实验检测，得到了表面增强拉曼信号强度随啶虫脒溶液浓度的变化规律。通过密度泛函理论模拟计算啶虫脒的振动模型，确定了啶虫脒的主要特征峰，以及各特征峰所对应的振动模式。结果表明，啶虫脒溶液各个特征峰信号随着啶虫脒溶液浓度的升高呈指数线性增强，并获得啶虫脒溶液的检测限为 1 ppm。 31528
毕业论文关键字：表面增强拉曼光谱   农残检测    啶虫脒   表面改性
Study of the SERS Performance of Silver Nanorod Array and Its Application in Acetamiprid Detection
Abstract:  The Surface-Enhanced Raman Scattering (SERS) technology with the characteristics of high sensitivity, low detection limit and rapid determination, is widely used in the field of biological detection, environmental monitoring, food safety and drug analysis. In this paper, surface modification is used for SERS active substrate, and the detection limit of acetamiprid is obtained, which  provides a new method and technology for pesticide residues. Firstly, diluted nitric acid was used for removing the silver oxide from the surface of the silver nanorod array substrate. The probe molecular 1,2-bis(4-pyridyl)ethylene (BPE) was detected by Raman spectrometer at the wavelength of 785 nm, and obtained the SERS signal of substrate cleaned by different concentrations. The results show that the detection limit of BPE can reach 10-8 M at the nitric acid concentration of 4.914 M, and its sensitivity is 100 times higher than the one without surface modification.  Next, the SERS signals of acetamiprid in different concentrations were detected, and the intensity changes with the concentration of acetamiprid solution. Simulation of acetamiprid vibration model by density functional theory determined the main characteristic peaks and the vibration mode of acetamiprid. The results show that the peak intensity of acetamiprid is increasing exponentially with the increase of the acetamiprid solution concentration, and the limit of detection is 1ppm.
Keywords:  Surface-enhanced Raman spectroscopy   Pesticide residues   Acetamiprid Surface modification
目录
1 引言  . 1
1.1  农药的介绍与使用现状  . 1
1.2  农药的研究方法及研究意义  . 2
1.3  本论文的主要研究工作  . 3
2 表面增强拉曼光谱  . 4
2.1  表面增强拉曼效应的发现  . 4
2.2  表面增强拉曼效应的基本原理  . 4
2.3  表面增强拉曼基底的制备方法  . 5
2.4  利用倾斜角沉积法制备 SERS活性基底的应用及进展  . 7
3 银纳米阵列基板的性能研究  . 10
3.1  银纳米阵列基板  . 10
3.2  实验材料  . 10
3.3  实验仪器  . 11
3.4  实验模型  . 11
3.5  实验步骤  . 12
3.6  结果与讨论  . 14
3.7  本章小结  . 18
4 杀虫类农药啶虫脒的表面增强拉曼光谱研究  19 新型银纳米阵列基板的SERS性能及农药啶虫脒检测研究:http://www.youerw.com/wuli/lunwen_27725.html