摘要：蜂胶有抗氧化，抗肿瘤，增强免疫等作用。蜂胶的水溶性差，生物利用度低。因此。在临床中蜂胶只用于美容养颜、愈合伤口等方面，而它的抗肿瘤作用很难得以利用。为了增加蜂胶在水中的溶解度，提高体内生物利用度，制备蜂胶乙醇提取物的碳酸钙纳米微球。本论文采用一步法制备蜂胶乙醇提取物@CaCO3复合纳米微球，通过优化反应时间，温度，转速，溶剂比，原料浓度等条件，得到vaterite含量高、球状形貌、粒径尺寸均一的复合纳米微球，并通过热重测试药物负载量。最优条件为：用浓度为0。05mol/L的碳酸钠和氯化钙与0。25g/mL的蜂胶乙醇提取液在室温，转速750rpm条件下反应8小时。得到球霞石含量95%以上，粒径为400nm，均一，分散的CaCO3@EEP复合微球。73755

毕业论文关键词： 蜂胶醇提取物；CaCO3；球状纳米微球； 一步法

Preparation and characterization of alcohol extracts from propolis

Abstract：Propolis has antioxidant, anti-tumor, enhance immunity and so on。 Propolis has poor water solubility and low bioavailability。 therefore。 In the clinical propolis is only used for beauty, healing wounds and so on, and its anti-tumor effect is difficult to use。 In order to increase the solubility of propolis in water and improve the bioavailability in vivo, the ethanolic calcium carbonate nanoparticles of propolis ethanol extract were prepared。 In this paper, the propolis ethanol extract @ CaCO3 composite nanometer microspheres was prepared by one-step method。 By optimizing the reaction time, temperature, rotational speed, solvent ratio and raw material concentration, the composite nanosheets with high vaterite content, spherical morphology and particle size were obtained。 Microspheres and tested for drug loading by thermogravimetry。 The optimum conditions were as follows: the reaction was carried out with sodium carbonate and calcium chloride at a concentration of 0。05 mol / L with 0。25 g / mL propolis ethanol extract at room temperature and at 750 rpm for 8 hours。 And the microspheres with homogeneous and dispersed CaCO3 @ EEP were obtained。

 KEY WORDS:  Propolis alcohol extract; CaCO3; spherical nanospheres; one-step method

目   录

1 概述 1

1。1   研究背景 1

1。2   文献综述 1

1。2。1  蜂胶的物理性质 2

1。2。2  蜂胶的成分 2

1。2。3  蜂胶的药理作用 2

1。4  纳米碳酸钙材料 4

1。4。1  碳酸钙的制备方法 5

1。4。2晶型调控剂对碳酸钙的影响 6

1。5研究依据及目的 6

2 蜂胶/碳酸钙复合纳米微球的制备及性能研究 8

2。1药品与仪器 8

2。1。1药品与试剂 8

2。1。2仪器 8

2。2常用仪器原理、操作方法及计算 8

2。3实验步骤 10

2。3。1 不同浓度蜂胶醇提取液对碳酸钙纳米微球的影响 10

2。3。2  不同反应时间对CaCO3@EEP复合纳米微球晶型的影响 11