摘要本文设计并制备了一种可注射型微球支架。该微球通过壳聚糖乳化交联制得，所得微球颗粒小，形态规整，表面光滑。研究了各制备参数对微球形貌、尺寸、粒径分布等方面的影响。可通过调整相关参数，对所制备微球的粒径、尺寸分布、分散度进行控制，以符合预期要求。研究了微球搭载BSA后形貌尺寸等方面的变化，测试了微球的释放性能与降解能力。研究发现，微球形成了BSA缓释体系，能有效控制BSA的释放速度，使BSA浓度较长时间的文持在一个稳定的范围内。微球在释放前期能保持规整的形貌，可形成一定机械强的支架，在后期又能逐步自行降解。这种壳聚糖微球有望促进骨细胞的依附和增值，可在骨组织修复方向得到应用。21838
关键词 微球支架 乳化交联 制备参数 骨组织修复
毕业论文设计说明书（论文）外文摘要
Title Preparation and Performance of injectable microspheres Polysaccharide
Abstract
In this article, injectable microspheres were designed and prepared. The chitosan microspheres was prepared by crosslinking emulsion, resulting microspheres small particles, regular shape, smooth surface. Study the effects of various parameters on the preparation for aspect of microspheres morphology, size, size distribution and the like. Adjust the parameters during the microspheres preparation, the particle size, size distribution, dispersion is controlled to meet the expected requirements. Study the change of microspheres after loading Bovine Serum Albumin and test the release properties of the microspheres and degradation. Research shows that BSA delivery system can be built by microspheres. BSA rate of release can be controlled effectively, the concentration of BSA in a long time to maintain a stable range. BSA concentration could be maintained in a stable range for a long time. In the early-release microspheres can remain intact morphology, can form a certain mechanical strength of the stand, but also the gradual degradation of their own in the late. This chitosan microspheres are expected to promote bone cell attachment and appreciation, can be applied in the direction of the bone tissue repair.
Keywords Microspheres stand，Emulsifying crosslinked， Parameters of Preparation，Bone tissue repair
目 次
1 绪论 1
1.1 微球支架 1
1.2 多聚糖的定义及简介 1
1.2.1 海藻酸钠 2
1.2.2 透明质酸 2
1.2.3 壳聚糖 3
1.3 CTS微球的制备方法简介 3
1.3.1 乳化交联法 3
1.3.2 离子凝胶法 3
1.3.3 中和沉淀法 4
1.3.4 喷雾干燥法 4
1.3.5 复凝聚法 4
1.3.6 溶剂蒸发法 4
1.4 CTS的交联反应 5
1.5 课题提出 5
2 实验部分 6
2.1 试剂与仪器 6
2.2 空白壳聚糖微球与载BSA微球的制备 6
2.3 壳聚糖微球的形貌 7
2.4 BSA标准曲线的绘制 7
2.5 载BSA微球的释放 8
3 结果与讨论 8
3.1 乳化交联制备壳聚糖微球 8
3.1.1 油水比(o/w)的影响 8
3.1.2 转速的影响 10 可注射型多聚糖微球的制备及性能研究:http://www.youerw.com/cailiao/lunwen_14231.html