摘要本文设计和合成了一种可用与抗凝血的肝素/明胶复合微球。该复合微球采用了利用化学接枝法，以氰基硼氢化钠为交联剂，利用明胶的氨基和肝素的醛基化学键结合得到，本文从包封率，载药量，微观形貌，粒径分布，缓释情况等方面研究了肝素/明胶复合微球，研究发现：与肝素化学接枝后的明胶微球具有理化性质稳定，表面形貌较为光滑的特点。化学接枝法得到的肝素/明胶复合微球，在100：1的比例下包封率和载药量都较高。这种微球有望提高肝素药物的利用率。可作为一种潜在的药物微球得到应用。23544
关键词 肝素 明胶微球 化学接枝
毕业设计说明书（毕业论文）外文摘要
Title Preparation and properties of gelatin/heparin composite microspheres
Abstract
In this aticle,A method used to synthesise gelatin/heparin composite microspheres which is available to anticoagulate was designed. The composite microspheres using chemical grafting method with sodium cyanoborohydride as the crosslinking agent. We have demonstrated a chemical grafting approach toward composite microspheres using sodium cyanoborohydride as a cross-linking agent and amino groups of gelatin combining with carboxyl group in the heparin via nucleophilic addition. The paper researches systematically heparin/gelatin composite microspheres from various aspects such as envelopment rate, drug loading, microtopography, particle size distribution, slow release performance,etc.This research suggest that gelatin microspheres with heparin is well stable of physical and chemical properties and smooth surface. Heparin / gelatin composite microspheres obtained by chemical grafting achieve a large encapsulation efficiency and ratio of loading drug in the ratio of 100:1.Encapsulation efficiency and drug loading are higher. It is expected to improve the utilization of the microspheres of the drug heparin.It will be used as a potential drug microspheres.
Keywords Heparin Gelatin microsphere Chemical grafting
目 录
第一章 绪论 1
1.1 微球的研究进展 1
1.1.1 微球分类 1
1.1.2 微球的制备 1
1.2 明胶作为药物载体材料的研究 3
1.2.1 明胶在医药学中的应用 4
1.3 低分子肝素的作用机理及研究进展 5
1.3.1 低分子量肝素的药理作用 6
1.4 课题的提出 7
第二章 实验部分 9
2.1 实验试剂及实验设备 9
2.2 明胶微球的制备与表征 9
2.2.1 明胶微球的制备 9
2.2.2 明胶微球的表征 10
2.3 肝素/明胶微球的制备及其表征 10
2.3.1 肝素/明胶微球的制备 10
2.3.2 肝素／明胶复合微球的表征 10
第三章 结果与讨论 13
3.1 微球降解性能测试 13
3.2 肝素/明胶复合微球的降解 14
3.3 药物载药量和包封率测定 15
3.4 微球的微观形貌 16 明胶/肝素复合微球的制备及性能研究:http://www.youerw.com/cailiao/lunwen_16657.html