荧光探针法测定烷基醇酰胺非离子型表面活性剂临界胶束浓度研究

摘要：以椰油酸二乙醇酰胺、芘(Py)、二苯甲酮、丙酮、氯化钠、乙醇和丙三醇为原料，配制含有芘的烷醇酰胺非离子型表面活性剂(椰油基二乙醇酰胺)溶液，通过稳态荧光探针光谱法测定其临界胶束浓度。根据芘发射光谱中芘的荧光强度比(I374/I384)随表面活性剂水溶液浓度的变化而变化，激发光谱中其荧光强度比(I338/I333)随表面活性剂水溶液浓度变化而变化，测定椰油酸二乙醇酰胺(6501)非离子型表面活性剂的CMC。结果两种光谱得到的CMC一致，为5×10-5mol/L。同时，研究了外部添加剂(无机盐和一元醇)对椰油酸二乙醇酰胺非离子型表面活性剂CMC的影响及其原因。结果表明两种外部添加剂对6501表面活性剂的CMC均有不同影响。当猝灭剂的浓度范围在0.04~0.07mmol/L时，用稳态荧光探针法测定了椰油酸二乙醇酰胺的胶束聚集数。结果表明，表面活性剂溶液浓度为5~10倍CMC，猝灭剂二苯甲酮浓度保持不变时，胶束聚集数N对表面活性剂溶液浓度具有依赖性，N随表面活性剂溶液浓度的增大而增大。5663
关键词：椰油酸二乙醇酰胺；非离子表面活性剂；荧光探针；临界胶束浓度；胶束聚集数
Determination of Critical Micelle Concentration(CMC) of Coco fattyacid diethanolamide by Steady-State Flourescence Probe Metherd
Abstract：Critical aggregation numbers of micelle(Nagg)of Alkanolamide nonionic surfactant was(Coco fatty acid diethanol amide) determined by steady-state fluorescence probe method in which pyrene was used as the fluorescence probe and benzophenone as thequencher．According to the change of fluorescence intensity ratio of pyrene I1／I3 with the change of surfactant concentration of aqueous solution,critical micelle concentration(CMC)was investigated by steady—state fluorescence probe and compared with that determined by surface tension method．CMC values for CEDA was 5.25×10-5 mol／L．Satisfactory results were obtained when the surfactant solution was prepared with saturated aqueous solution of pyrene and the optimum concentration of benzophenone was selected at 0.04-0.07 mmol／L．The experimental results showed that Nm increased linearly with the increase of the surfactant concentration in a range of 5—15 times CMC and Nm can be extrapolated from the N— C curve，which was 5．
KeyWords: Coco fatty acid diethanolamide，nonionic surfactant,fluorescence probe，critical micelle concentration,aggregation number of micelle
目录
1 前言   1
1.1烷醇酰胺的国内外研究现状   1
1.2荧光探针法测定表面活性剂临界胶束浓度的原理   2
1.2.1 临界胶束浓度的定义2
1.2.2 临界胶束浓度测定方法.2
1.2.3 荧光探针法测定临界胶束浓度的原理4
1.3胶束聚集数   5
1.4本课题的研究的目的和意义   5
2 实验部分   7
2.1 实验原料   7
2.2 实验仪器与设备   7
2.3 实验溶液的配制   8
2.3.1CMC测定所需个标准溶液的配制8
2.3.2胶束聚集数测定所需溶液的配制   9
2.4实验测定方法   10
     2.4.1不同浓度烷醇酰胺(6501)溶液中芘发射光谱测定10
     2.4.2不同浓度烷醇酰胺(6501)溶液中芘激发光谱测定.11
     2.4.3胶束聚集数N测定.11
3 实验结果与讨论   12
3.1烷醇酰胺类表面活性剂的荧光光谱   12
3.1.1不同浓度烷醇酰胺(6501)溶液中芘的荧光光谱   12
3.1.2发射光谱中I374/I384随6501浓度的变化曲线   13
3.2外部添加试剂对烷醇酰胺(6501)临界胶束浓度的影响   15 荧光探针法测定烷基醇酰胺非离子型表面活性剂临界胶束浓度研究:http://www.youerw.com/huaxue/lunwen_2790.html