摘要： 本实验论文成功制备了具有温度敏感性的聚乙烯亚胺，聚(N-异丙基丙烯酰胺)的磁性纳米颗粒，然后用叶酸进行修饰得到主动靶向药物载体，并选用抗癌药物阿霉素进行药物负载。通过IR技术对所制备的磁性纳米颗粒进行结构表征，X射线衍射实验（XRD）研究Fe3O4的包覆结果。研究了不同温度和不同pH值对药物释放的影响。结果表明，Fe3O4磁性纳米粒成功被聚合物所包覆；该载药系统的较低临界溶解温度（LCST）在45~47℃左右；该载药系统在较低的pH下药物释放率较大，且在LCST温度附近药物释放速率快。6416
关键词： 温敏性聚合物；纳米粒子；药物载体；较低临界溶解温度；药物释放
Preparation and properties study of the NIPAM-PEI polymer modified by folic acid
Abstract：In this study, the temperature-sensitive magnetic nanoparticles successfully synthesized by polyethyleneimine and poly(N-isopropylacrylamide) , were further modified by folic acid and obtained active targeting drug carrier .An anticancer drug, doxorubicin, is encapsulated into the drug carrier. The synthesized magnetic nanoparticles were characterized by IR techniques and the Fe3O4 coating results were studied by X-ray diffraction (XRD). The impacts of different temperatures and pH on the drug release were studied. The X-ray diffraction (XRD) results showed that Fe3O4 magnetic nanoparticles were successfully coated by polymer. The lower critical solution temperature (LCST) of the drug delivery system was at about 45 ° C to 47 ° C. In the lower pH, the amount of drug release of the drug delivery system was larger and near the LCST Temperature, the rate of drug release was higher.
Key Words：Temperature-sensitive polymer; Nanoparticles; Drug carrier; Lower critical solution temperature; Drug release
目录
1. 绪论 1
1.1 纳米技术与纳米载药系统 1
1.2 聚N—异丙基丙烯酰胺简介 1
1.2.1 聚N—异丙基丙烯酰胺的温敏机理 2
1.2.2 聚N—异丙基丙烯酰胺的应用 2
1.2.3 聚N—异丙基丙烯酰胺的研究状况 4
1.3 聚乙烯亚胺概述 5
1.3.1 聚乙烯亚胺的特性 5
1.3.2 聚乙烯亚胺作为非病毒载体的缺陷 6
1.3.3 聚乙烯亚胺的研究现状 6
1.4 叶酸概述 6
1.4.1叶酸靶向的原理 7
1.4.2叶酸的应用及研究状况 7
1.5 本课题研究目的及意义 8
2. 实验内容 10
2.1 实验原料与设备 10
2.1.1 实验原料 10
2.1.2 实验设备 11
2.2 实验方法 11
2.2.1 叶酸修饰的磁性纳米粒子的合成 11
2.2.2 阿霉素的负载与释放 12
2.2.3 纳米粒子的红外光谱检测 13
2.2.4 纳米粒子的粒径测量 13
2.2.5 制作两种溶剂的溶胀曲线 13
2.2.6 同一温度下两种不同pH的体外释放 13
2.2.7 同一pH值下两种不同温度的体外释放 14
2.2.8 不同材料在相同温度和pH条件下的体外释放 14
3. 结果与讨论 15
3.1 纳米粒子的表征 15
3.3.1 红外光谱检测 15
3.3.2 纳米粒子的XRD分析 16
3.2 聚合物溶液的LCST测定 18
3.3 Fe3O4@PNIPAAEF纳米粒子在不同溶剂下溶胀性的比较 21
3.4 体外释放 22
3.4.1 标准曲线 22 叶酸修饰NIPAM-PEI聚合物的研制及性能研究:http://www.youerw.com/huaxue/lunwen_3949.html