1、氧化石墨烯(GO)是根据Hummer的方法由石墨粉提取
2、DNA 的荧光检测。
3)创新点:
现已有大量文献报道关于某些金属离子可以和一些错配碱基形成特殊的稳定结构的研究。自然碱基对与这种由金属离子介导形成的碱基对的区别在于,前者通过氢键结合以形成稳定的结构,后者通过金属离子来稳定结构。目前比较常用到的金属介导的碱基对特殊结构有胞嘧啶(C)与金属离子(Ag+)形成的C-Ag+-C结构和胸腺嘧啶(T)与金属离子(Hg2+)形成的T-Hg2+-T特异性结构。本文利用Hg2+介导T碱基配对形成的T-Hg2+-T特异性结构。该结构的形成不仅可以使两条富含T碱基的DNA单链形成稳定的双链结构,而且还可以使单链DNA的结构发生变化,使其折叠成双链。DNA和金属离子的这种特殊性质为传感器的研究提供了一种很好的思路。
课题进度安排:
07-08 查阅资料,并完成预实验
08-11 完成实验
04-05 完成实验撰写
主要参考文献:
[1] Borjesson J, Isaksson M, Mattsson S. X-ray fluorescence analysis in medical sciences: a review [J]. Acta Diabetologica, 2003, 40 (1): 39-44.
[2] Hanley Q S, Murray P I, Forde T S. Microspectroscopic fluorescence analysis with prism-based imaging spectrometers: review and current studies [J]. Cytometry A,2006,69(8):759-766.
[3] Li Z, Wei Q, Yuan R, et al. A new room temperature ionic liquid 1-butryl-3-trimethylsilylimidazolium hexafluorophosphate as a solvent for extraction and preconcentration of mercury with determination by cold vapor atomic absorption spectrometry[J]. Talanta, 2007, 71: 68-72.
[4] Zheng C B, Li Y, He Y, et al. Photo-induced Chemical vapor generation with formic acid for ultrasensitive atomic fluorcscence spectrometric determination of mercury: potential application to mercury speciation in water [J]. Journal of Analytical Atomic Spectrometry, 2005, 20(8): 746-750.
[5] Ahmed M J, Alam M S. A rapid spectrophotometric method for the determination of mercury in environmental, biological, soil and plant samples using diphenylthiocarbazone[J]. Journal of spcctroscopy, 2003, 17(1): 45-52.
[6] Zheng H, Qian Z H, Yuan F F, et al. Switching the Recognition Preference of Rhodamine B Spirolactam by Replacing One Atom: Design of Rhodamine B Thiohydrazide for Recognition of Hg(11) in Aqueous Solution [J], Organic Letters, 2006, 8(5): 859-861.
[7] Wang S P, Forzani E S, Tao N J. Detection of Heavy Metal Ions in Water by High-Resdution Surface Plasmon Resonance Spectroscopy Combined with AnodicStripping Voltammetry [J]. Analytical Chemistry, 2007, 79: 4427-4432.
废水中重金属汞离子荧光法检测开题报告(2):http://www.youerw.com/kaiti/lunwen_55631.html