摘要大豆蛋白经超声波辅助酶法水解可以得到高水解度多肽，此种多肽有α-葡萄糖苷酶抑制活性。实验从多肽的性质出发，利用多肽的离子强度，疏水性筛选能有效分离多肽，并能得到高于原液α-葡萄糖苷酶抑制活性组分的树脂。筛选出了大孔吸附树脂DA201-C型和732阳离子交换树脂效果最好。并且再使用凝胶色谱和高效液相色谱纯化得到了高纯度肽，建立体外模拟人体消化模型，发现多肽具有良好稳定性，具有开发为产品的潜力。86842

实验使用25%，50%，75%，100%的酒精将大孔吸附树脂吸附的肽分为四个疏水性不同的组分，25%酒精洗脱的组分具有最好的α-葡萄糖苷酶抑制活性，为40。77%。使用2%的氨水可以将732阳离子交换树脂吸附的肽分为2个组分，峰1具有最好的α-葡萄糖苷酶抑制活性，为56。33%。对两种树脂的最优组分分别作稳定性试验，稳定性都比较好。

实验再经过凝胶色谱，高效液相色谱对两种树脂的最优组分进行分离，最终得到组分基本单一色谱峰，对色谱峰的研究表明大孔吸附树脂可以分离出4。92%的α-葡萄糖苷酶抑制率为50。70%的高纯度肽，而732阳离子交换树脂可以分理出0。27% α-葡萄糖苷酶抑制率为63。32%的高纯度肽，并且两种肽生理稳定性良好。

实验通过对大豆肽得率的分析，发现对于大豆降血糖肽的产品化，两种树脂的分离具有不同指导意义。若使用水解法生产大豆降血糖肽产品，大孔树脂的分离实验有好的效益，若使用蛋白质工程法生产大豆降血糖肽，则732阳离子交换树脂分离出的大豆肽更有研究价值。

毕业论文关键词：活性肽  大孔吸附树脂  α-葡萄糖苷酶    732阳离子交换树脂

Abstract The soybean protein can be hydrolyzed by ultrasonic assisted enzymatic hydrolysis to obtain a high degree of hydrolysis polypeptide, which has the activity of Lowering blood sugar。 Experiment using the ionic strength of the polypeptide, hydrophobic screening effective separation of peptides, and can get higher inhibitory activity polypeptide than the original polypetide。 Then macroporous adsorption resin DA201-C and 732 cation exchange resin was screened out。 High purity peptides were purified by gel chromatography and high performance liquid chromatography。 The model was established to simulate the human body digestion in vitro。 The peptides were found to have good stability and potential for development in industry。

The experiments used 50%, 25%, 75%, 100% alcohol to adsorb the macroporous adsorption resin to the adsorption of the peptides into four hydrophobic groups, and the 25% ethanol eluted fractions had the best inhibitory activity, 40。77% 。 The use of 2% ammonia water can seperate 732 cation exchange resin adsorption of the peptides into 2 components, peak 1 has the best alpha - Grape glycosides inhibitory activity, 56。33%。 The optimal composition of the two kinds of resins were used for stability test, and the stability was good。

Experiment using gel chromatography, high performance liquid phase chromatography get the two resin optimal group separated, and finally get the basic single chromatographic peaks of the components, the study of chromatographic peaks showed that macroporous adsorption resin can  isolated high purity of the peptide 4。92% alpha glucosidase inhibitory rate 50。70%, and 732 cation exchange resin can get 0。27% high purity of the peptide alpha glucosidase inhibitory rate of 63。32%, and two peptide physiological stability good。

Through analyzing the yield of soybean peptides, it is found that the separation of the two kinds of resins has different guiding significance for the production of soybean protein。 If using hydrolysis method produce hypoglycemic peptide products and macroporous resin separation experiments have good benefits, if using protein engineering method for production of soybean peptide for lowering blood sugar, 732 cation exchange resin separation of soybean peptide has more research value。 超声波辅助酶法制备大豆降血糖肽的分离纯化研究:http://www.youerw.com/shengwu/lunwen_116823.html