摘要：近年来，细菌的耐药谱不断扩大，抗菌药物的防治效果下降，且易在禽体内形成残留，影响了人类健康和公共卫生安全植物源性生物活性物质与化学合成物质相比，具有对人畜毒性小、不易产生耐药性、副作用少、环境污染小和残留低等优点，已经引起全球学者的广泛关注。综合上述，基于我国具有丰富的油掉资源，而且利用仅仅限于油樟的粗提物，且许多油樟油中单体物质未被充分利用，因此本研究在前期实验室研究的基础上，拟以樟树叶与天竺桂叶片所提取的植物精油作为研究对象，研究其对目前在兽医临床上危害较大大肠杆菌(细菌)的抗菌活性，以及对人体危害大的黑曲霉（真菌），蹄选得到具有较好抗此两类微生物活性的天然药物。本文首先简单介绍了樟树与天竺桂，然后对樟树与天竺桂抑菌物质和抑菌效果进行了重点阐述，得出天竺桂精油对细菌的抑制作用较强烈。而樟树叶精油却对真菌的抑制作用比较强烈，以期对以后的植物精油抑菌研究提供一定的参考价值。22051
毕业论文关键词：樟树叶；天竺桂；抑菌效果；提取
Camphor tree leaves, such as plant bacteriostat qualitative extraction and bacteriostatic effect research
Abstract: In recent years, Growing bacterial resistance spectrum, Control effect of antimicrobial drugs fall, And easy in vivo formation of residual poultry, affecting human health and public health security of bioactive substances of plant origin compared with chemical synthesis matter, has little toxicity to human and animal, not easy the creation bears the medicine, less side effects and low environmental pollution, and low residual, has caused extensive concern of the global scholars. Comprehensive above, based on our country has rich oil resources, and use only in crude extract oil, camphor, and many oil monomer in the camphor oil substances underused, therefore on the basis of preliminary laboratory studies, this study intends to main chemical compositions of the oil, camphor oil (1, 8 - eucalyptus oil, a - terpineol, pine oil, thin - 4 - alcohol, Y - pine oil refining) as the research object, research on the harm in veterinary clinic larger escherichia coli bacterium antibacterial activity, with good resistance to e. oli hoof pick activity of natural medicine, and study its antibacterial mechanism, laying the groundwork for hoof choose si standard drugs. This paper first introduces the camphor tree, then the camphor tree focuses on antibacterial material and antibacterial effect, Cinnamomum japonicum oil paint on bacterial inhibition is relatively strong. The camphor tree leaves essential oil has a strong inhibitory effect on fungi. in order to later of plant essential oil bacteriostatic research provides certain reference value.
Keywords：Camphor leaves; Bacteriostatic effect; Extraction; research
目录
1 绪论 - 1 -
1.1 简介 - 1 -
1.2 本课题的国内外研究进展 - 1 -
1.2.1 国内研究进展 - 2 -
1.3 国外的研究进展 - 3 -
1.4 本课题的研究目的和意义 - 3 -
1.4.1 精油的大类 - 3 -
1.4.2 植物精油的抑菌机理 - 3 -
1.4.3 研究目的和意义 - 4 -
2.实验材料和方法 - 5 -
2.1 材料 - 5 -
2.1.1 仪器与试剂 - 5 -
2.1.2 材料 - 6 -
2.1.3 供试菌种与培养基 - 6 -
2.2 实验方法 - 6 -
2.2.1 樟树叶等植物叶精油的提取 - 6 -
2.2.2 大肠杆菌菌悬液的制备 - 6 - 樟树叶等植物抑菌物质的提取与抑菌效果的研究:http://www.youerw.com/shengwu/lunwen_14516.html