摘要：合理的主茎节数是构成大豆[Glycine max(L.)Merr]理想株型的重要因子，也是高产潜力的遗传基础，但其遗传机理及其育种价值仍需深入研究。本研究以代换系 CSSL3228×南农 1138-2 构建的447 份 F2:3家系群体为试验材料，运用植物数量性状主基因+多基因混合遗传模型(SEA-F2)对主茎节数性状进行遗传分析，并对得到的 4 个主茎节数候选基因(Glyma.13g233600，Glyma.13g231000，Glyma.13g231100和Glyma.13g252900)进行不同时期不同组织中的表达研究。结果表明：大豆主茎节数主要受 2 对加性-显性主基因+加性-显性多基因控制，第 1、2 对主基因的加性效应分别为 8.83、4.93，主基因遗传率为 68.61%。4 个基因中 Glyma.13g233600 主要在叶片和茎尖中高表达，在代换系 CSSL3228 和南农 1138-2 初花期茎中表达具有显著差异。因此，Glyma.13g233600 可能参与大豆主茎节数生长发育调控，这将为大豆主茎节数调节基因克隆和分子标记选择育种发展奠定基础。 27427
毕业论文关键词：大豆；主茎节数；遗传分析；候选基因；
Genetic Analysis and Candidate Genes Analysis of the Soybean’s Main Stem Node Numbers Abstract: Suitable node numbers of main stem is an important factor in the ideal plant type of soybean and the genetic basis of soybean high-yield potential. But its genetic mechanism and breeding potential still need to be studied deeply. In this study, we used 447 F2:3 families which were constructed by using the intercropping system of CSSL3228×Nannong 1138-2 as the experimental material. Apply the multiple gene hybrid genetic model (SEA-F2) to analysis the main stem soybean nodes’ heredity and study the expression of four candidate genes in soybean’s three tissues and five growth periods. The results show that the main stem nodes numbers were mainly controlled by two pairs of additive-dominant main genes plus additive-dominant polygenes, the additive effects of the first and second main genes were 8.83 and 4.93, respectively. And the main gene heritability was 68.61%. Out of the four candidate genes, Glyma.13g233600 was significantly higher than the others in leaves and shoots, and expressed dramatic difference during the early blooming period between substitutional line CSSL3228 and Nannong 1138-2. So we conclude that Glyma.13g233600 might take part in regulating the growth and development of soybean’s main stem node numbers and mainly worked in the early blooming period. This study could lay the foundation for the development of further gene cloning and molecular marker selection breeding about soybean’s main stem node numbers. Key words: Soybean; Number of nodes on main stem; Genetic analysis; Candidate gene
目 录
摘要 .. 1
关键词 . 1
Abstract 1
Key words .. 1
1 引言 . 2
1.1 大豆主要株型性状及遗传育种研究进展 2
1.1.1 根部性状研究 . 2
1.1.2 叶片形态及遗传 2
1.1.3 茎秆相关性状研究进展 3
1.1.4 株型育种研究及进展 . 3
1.2 大豆主茎节数的遗传特性研究 . 3
1.3 大豆主茎节数 QTL定位研究 4
1.4 作物株型相关基因的克隆及技术 4
1.4.1 株型相关基因的克隆 . 4
1.4.2 SNPs 检测 .. 4
1.4.3 实时荧光定量 PCR 技术 .. 5
1.5 本研究的目的与意义 .. 5
2 材料与方法 . 6
2.1 试验材料 . 6
2.1.1 试验材料 6
2.1.2 田间调查及取样 6
2.1.3 试验试剂及仪器 6
2.2 试验方法 . 6 大豆主茎节数的遗传分析及候选基因分析:http://www.youerw.com/shengwu/lunwen_21906.html