摘 要：类黄酮（Flavonoid）是一类广泛存在于植物器官中的多酚类物质，由于在生物体抗衰老、植物抗逆性等生命活动中具有重要作用，近年来被广泛研究。黄烷酮-3-羟化酶（Flavanone 3-hydroxylase，F3H）是类黄酮生物合成过程中的关键酶。本文从NCBI蛋白数据库中检索并下载了10条小麦（Triticum aestivum）黄烷酮-3-羟化酶序列，并采用生物信息学方法对这些蛋白序列进行了多序列比对，根据同源度将其分为3个家族，并对3个家族的成员进行了磷酸化、亚细胞定位、保守域、信号肽、紊乱区球形区、二级结构预测、三级结构建模、理化性质和进化分析等方面的研究分析。结果表明：在小麦黄烷酮-3-羟化酶家族成员中，Ⅰ类黄烷酮-3-羟化酶与Ⅱ类黄烷酮-3-羟化酶和Ⅲ类黄烷酮-3-羟化酶的各种理化性质、二级结构等存在明显差异。小麦黄烷酮-3-羟化酶与大麦的黄烷酮-3-羟化酶相似性较高，进化距离较近。研究结果为进一步从分子水平深入研究小麦类黄酮提供了重要的理论基础。30340
毕业论文关键词：小麦；类黄酮 ；黄烷酮-3-羟化酶；研究进展；生物信息学
The Bioinformatics Analysis And Research Progress of Flavanone 3-hydroxylase（F3H）of Wheat
Abstract: Flavonoids (Flavonoid) is a widely present in the plant organs polyphenols, as an important role in aging organisms, plant resistance and other life activities, has been extensively studied in recent years. Flavanone 3-hydroxylase (flavanone 3-hydroxylase) is a key enzyme in the flavonoid biosynthesis, protein-containing Fe element within the family, its research related to the flavonoid synthesis. This article is retrieved from the NCBI protein database and downloaded 10 wheat (Triticum aestivum) flavanone 3-hydroxylase sequences and bioinformatics methods for these protein sequences were multiple sequence alignment, according to the degree of homologyIt will be pided into three families, and members of the three families were phosphorylation, subcellular localization, conserved domains, signal peptide, disordered region spherical zone, secondary structure prediction, tertiary structure modeling, physical and chemical properties and evolution forecasting and analysis aspects of analysis. The results showed: wheat flavanone 3-hydroxylase family members, class Ⅰ flavanone 3-hydroxylase and Ⅱ class flavanone 3-hydroxylase and Ⅲ class flavanones -3- significantly different physicochemical properties, secondary structure hydroxylase like. Flavanone 3-hydroxylase of Wheat and Barley have high similarity, closing evolutionary distance. The research results provide a theoretical basis for further research.
Key words： Flavonoids; Triticum aestivm; Flavanone 3-hydroxylase; Advances; Bioinformatics
目 录
摘 要 1
引 言 2
1类黄酮及黄烷酮-3-羟化酶研究进展 2
1.1 类黄酮的研究进展 2
1.2 黄烷酮-3-羟化酶的研究进展 3
2 类黄酮生物信息学分析 3
2.1 材料和方法 3
2.1.1 小麦类黄酮黄烷酮-3-羟化酶序列的获得 3
2.1.2 小麦类黄酮黄烷酮-3-羟化酶同源性比较和聚类分析 3
2.1.3 基本理化性质分析 3
2.1.4 磷酸化、亚细胞定位、跨膜结构域、保守域、信号肽及紊乱区分析 3
2.1.5 二级结构预测 4
2.1.6 三级结构预测 4
2.1.7 进化分析 4
2.2 结果与分析 4
2.2.1 小麦类黄酮黄烷酮-3-羟化酶同源性比较和聚类分析 4
2.2.2 小麦黄烷酮-3-羟化酶理化性质分析 6
2.2.3 小麦黄烷酮-3-羟化酶磷酸化分析 7 小麦类黄酮的研究进展及生物信息学分析:http://www.youerw.com/shengwu/lunwen_26003.html