油菜A20/AN1锌指蛋白挖掘与鉴定

摘要：A20/AN1锌指蛋白普遍存在于真核生物中，是一类庞大的转录调控因子家族，通过与核酸或蛋白质结合行使植物功能，参与多个生理过程。近年来研究发现，植物A20/AN1锌指蛋白在胁迫回应引起的信号传导过程中发挥着重要作用。本文利用生物信息学方法和技术，对油菜A20/AN1锌指蛋白进行分析，以了解其相关结构和功能信息。生物信息学分析显示该蛋白位于细胞质中，具有磷酸化位点，参与细胞转录调控。油菜A20/AN1锌指蛋白被分为3类，三类A20/AN1锌指蛋白氨基酸长度、等电点差异不大，且序列中20种常见氨基酸含量接近；三种蛋白的二级结构均以无规则卷曲为主，其次是α螺旋，延伸链和β-转角比例较少。本文主要通过对油菜A20/AN1锌指蛋白进行挖掘与鉴定，以获得有功能的相关蛋白基因，对作物耐逆性进行遗传改良，提高作物的耐逆能力。35775
毕业论文关键字：油菜；A20/AN1锌指蛋白；序列分析；生物资讯学
Mining and Identification of A20/AN1 Zinc Finger Protein in Brassica napus L
Abstract: A20/AN1 zinc finger protein generally existing in eukaryotic, is a kind of large family of transcription regulatory factors, through combination with nucleic acid or protein, they conduct exact function , involving in multiple physiological processesin plant. The study in recent years found that, the A20/AN1 zinc finger protein plays an important role in the process of signal transduction in the stress response. Using bioinformatics methods and techniques, we analyzed the A20 / AN1 zinc finger protein from rapeseed, to understand its structure and function of the relevant information. The results showed that the protein is located in the cytoplasm, with different phosphorylation site, they participate in cell transcriptional regulation. Results also showed that the A20 / AN1 zinc finger proteins are pided into three categories, they have small difference in acids length, isoelectric point, with 20 common amino acids composition; The secondary structure OF three kinds of protein mainly include random coil, then alpha helix, extending chain and beta-turns. In this article, we mainly mined and identified the A20/AN1 zinc finger protein of Brassica napus L, for obtaining their function information, improving and increasing crop resistance to stresss.
Key words: Rapeseed; A20/AN1 zinc finger protein; Sequence analysis; Bioinformatics
目    录
摘要    1
引言    2
1材料和方法    2
1.1材料来源    3
1.2 A20/AN1锌指蛋白的生物信息学分析    3
2结果和分析    3
2.1 油菜A20/AN1锌指蛋白序列获取与分析    3
2.2 A20/AN1锌指蛋白的亚细胞定位结果    4
2.3 蛋白氨基酸序列同源比对和进化分析    5
2.3.1蛋白氨基酸序列同源比对    5
2.3.2 蛋白氨基酸序列构建进化分析    6
2.4 蛋白质跨膜区、磷酸化分析    7
2.5 蛋白的二级结构及保守域分析    8
2.6 蛋白紊乱区、球蛋白区预测    9
3结论与讨论    9
参考文献    11
致谢    13
油菜A20/AN1锌指蛋白挖掘与鉴定引言：油菜（Brassica napus L.）属于十字花科，是世界四大主要油料作物之一，我国油菜产量居世界油料作物产量的第三位。我国大多数地区均可以种植油菜，但低温寒害、干旱、风和病害等逆境胁迫严重影响了油菜生产产量。拟南芥隶属于十字花科，广泛用于植物遗传学、发育生物学和分子生物学的研究，而且对它的研究比较透彻，全部基因组测序已经完成。油菜的大部分基因与拟南芥的基因存在这很高的同源性，所以可以利用拟南芥的相关蛋白序列来分析油菜同源序列。油菜A20/AN1锌指蛋白挖掘与鉴定:http://www.youerw.com/shengwu/lunwen_33882.html