摘要本研究的目的是保护与利用秦川牛遗传资源的多样性，为分子育种的开展奠定基础以及大幅提高秦川牛的生产性能提供依据。通过PCR-SSCP和PCR-RFLP及测序技术，研究了秦川牛基因组中bta-miRNA-101的遗传多态性及其群体遗传结构特征，利用统计方法分析了其多态基因类型与秦川牛生长发育性状的联系，以寻找秦川牛生长发育性状选种的分子标记，为秦川牛优秀生长发育性状的利用提供参考。实验总共提取了124个血样进行分析，发现了bta-miRNA-101存在2个多态位点，其中P2位点的突变对秦川牛体高和十字部高显著相关（P<0.05），P6位点的突变对秦川牛体长显著相关（P<0.05）。推测其对秦川牛的体尺性状有显著影响，可作为秦川牛分子育种的辅助标记。41949

毕业论文关键词：秦川牛  bta-miRNA-101  PCR-SSCP  遗传多态性  体尺性状

Study on the relationship between the genetic polymorphism in bta - miRNA - 101 and growth traits  of qinchuan cattles

Abstract

The purpose of this study is to protect and use the persity of qinchuan cattles genetic resources .TO lay a foundation for the development of molecular breeding and greatly improve the production performance of qinchuan cattles. By PCR - SSCP and PCR - RFLP and sequencing technology, we can study on the genome genetic polymorphism and its population genetic structure characteristics of the bta -  miRNA - 101 in qinchuan cattles. Using the least square method to analyze the relationship between its polymorphism gene types and qinchuan cattles growth traits, to search selective molecular markers of qinchuan cattles growth traits. Through these methods,we can provide important basis for the excellent growth traits in molecular breeding of qinchuan cattles. This experiment took a total of 124 blood samples to analyze, and probably discovered more than 30 samples of polymorphic loci. Amoung all these polymorphic loci,the mutation of P2  site signifigantly contribute to qinchuan cattles’s body height,and cross department.(P<0.05) ,the mutation of P6 site signifigantly contribute to qinchuan cattles’s body length. (P<0.05). We can speculate that it has a significant impact on body measurements traits of qinchuan cattles and can be used as a auxiliary marker on the molecular breeding of them.

Key words: Qinchuan cattles  Bta - miRNA - 101  The PCR - SSCP  Genetic polymorphism  Body traits

目录

摘  要 I

Abstract II

图清单 IV

表清单 IV

1 前言 1

2 材料与方法 2

2.1提供材料 2                                                    

3 实验方法与过程 3

3.2琼脂糖凝胶电泳检测其DNA 3

3.3引物设计 3

3.4 PCR-SSCP分析 4

3.5图像分析 6

4 结果与分析 6

4.1秦川牛基因组样品的检测 6

4.3 DNA测序及序列分析 7

4.4秦川牛bta-miRNA-101基因P2和P6位点遗传结构分析 8

4.5秦川牛bta-miRNA-101基因多态性与体尺性状的关系 9