摘要: 本课题以青稞为原料,利用生物酶和微生物发酵技术获得代谢产物,对代谢产物进行分析鉴定,确定其生物活性,进而开发相关产品。制备过程包括糊化,液化,糖化和发酵。利用DNS法测定还原糖和刚果红法测定β-葡聚糖的含量来确定各个过程中各因素对于产物的影响,优化其制备条件。实验结果表明:制备过程中,糊化温度为60℃,时间为10钟。液化加酶量为0.15%,温度为75℃,时间30分钟。糖化加酶量为0.15%,pH值为4.5,温度为55℃,时间为2h。酵酵母菌加入量为0.4%,温度为30℃,时间为8h。42727
毕业论文关键词: 青稞;糊化;发酵;面膜
Preparation and application of microbial fermentation product of highland barley
Abstract:This design using highland barley sa material, the use of enzyme and microbe fermentation technology for metabolites, analyze metabolites identified, determine its biological activity, and to develop related products.Preparation process including gelatinization, liquefaction, saccharification and fermentation.Using the DNS method for the determination of reducing sugar and Congo red method for determining the content of beta glucan to determine the effects of various factors on the product, in the process of the optimization of the preparation conditions. Experimental results show that the preparation process, the gelatinization temperature is 60 ℃, time is 10 minutes.Liquefaction with enzyme amount is 0.15%, the temperature is 75 ℃, 30 minutes.Saccharifying enzyme quantity 0.15%, pH value is 4.5, temperature is 55 ℃, time is 2 h.Yeast yeast addition amount is 0.4%, the temperature is 30 ℃, time is 8h.
Keywords : Highlandbarley ; Gelatinization ; Fermentation ; The mas
目录
1绪论 3
1.1青稞的概述 3
1.2 营养成分分析 3
1.3 青稞的应用现状 3
1.4本课题的研究目的和意义 4
2 实验内容 5
2.1 实验原料与设备 5
2.1.1 实验原料 5
2.1.2 实验所用设备 5
2.2 实验方法 6
2.2.1青稞浆的制备方法 6
2.2.2 DE值测定方法 6
2.2.3 β-葡聚糖含量的测定方法 7
3实验结果与讨论 8
3.1 糊化条件的确定 8
3.1.1糊化温度对青稞浆性质的影响 8
3.2 液化 10
3.2.1 不同液化温度对青稞浆性质的影响 10
3.2.2 不同淀粉酶加酶量对青稞浆性质的影响 12
3.2.3 不同液化时间对青稞浆性质的影响 13
3.3 糖化 15
3.3.1 不同糖化酶加酶量对青稞浆性质的影响 15
3.3.2不同糖化温度对青稞浆性质的影响 16
3.3.3 不同糖化时间对青稞浆性质的影响 17
3.3.4 不同PH对青稞浆性质的影响 18
3.4 微生物发酵 20
3.4.1 酵母菌的加入量 20
青稞微生物发酵产物的制备与应用:http://www.youerw.com/shengwu/lunwen_43252.html