摘要：芦丁是药物，它的物理形态是浅黄色针状结晶（水）。芦丁有很多的别名，例如芸香苷，芸香叶苷，芦丁芸香苷等。临床用于防治脑溢血、高血压、视网膜出血、紫瘢和急性出血性肾炎。芦丁的研究进展比较快，通过广泛采用现代的许多手段和方法,不仅可以提高芦丁的溶解速度同时也提高了芦丁的生物利用度,丰富了中药的基础性研究。这次我们主要是要研究芦丁在进入小鼠体内以后，芦丁的药代动力学研究，我们将用高效液相色谱法研究，观察芦丁在小鼠体内肾原药排泄的变化。我们这次会研究芦丁在尿中的分解情况，芦丁对尿的降解，芦丁在不同时间尿的降解率，以及芦丁最后排泄出体外的形式。这次研究我们希望给以后研究芦丁能提供更好的方法方式，更加方便的研究芦丁。49315

毕业论文关键词：芦丁、高效液相色谱（HPLC）、药代动力学、肾原药排泄

Kinetics of kidneys of mice after oral administration of rutin by the original drug excretion

Kewen College, Jiangsu Normal University, Xuzhou,Jiangsu,221116,China

Abstract:Rutin is a kind of medicine, it's a crystal (water), which looks like a pale yellow needle. Rutin also has many other names, such as yunxianggan, yunxiangyegan, ludingyunxianggan and so on. It can be used to prevent stroke, hypertension, retinal hemorrhage, peliosis and acute hemorrhagic nephritis. The development of rutin's research is so rapidly that we can use lots of modern methods and technique to enrich the basic study of traditional Chinese medicine. Improving rutin's solution rate and biological availability are very important. In this essay, we mainly to discuss rutin's pharmacokinetics study after runtin entering into the body of mice. We will use the High Performance Liquid Chromatography to observe rutin's renal excretion of technical change in mice's body. We also have to research rutin's decomposition situation and its degradation affect to the mice's urine. Rutin's degradation rate to the urine at different time and its form after excreting from the body are also included. We hope this research can help us find out a better and more convenient way to study rutin.

Keywords:Rutin; High performance liquid chromatography (HPLC); Pharmacokinetics; Renal excretion of the original drug

目录

前言 4

1 材料与方法 4

1.1供试材料 4

1.2芦丁的获得和储藏 5

1.3用高效液相色谱（HPLC）检测分析芦丁并建立相关标准曲线 5

1.4芦丁直接放入小鼠原尿中的回收率 5

1.5芦丁放入尿以后在不同时间的降解率 6

1.6不同时间所收集尿中芦丁的含量变化 6

2结果与分析 6

2.1芦丁标准品的HPLC色谱图 6

2.2芦丁标准品的标准曲线 7

2.3不同浓度芦丁直接放入尿中的回收率 7

2.4不同时间尿对芦丁的分解变化 8

2.5 不同时间所收集尿中的芦丁的含量变化 9

2.6芦丁标准品的HPLC色谱图及其标准曲线的分析 9

2.7不同浓度的芦丁直接放入尿中的回收率的分析 9

2.8不同时间尿对芦丁的降解变化的分析 10