利用转录组学研究甘薯响应黑斑病的分子机制

摘要甘薯不仅是世界上重要的粮食作物，也是重要的工业原料用作物。黑斑病是甘薯最为严重的三大危害之一，常年对甘薯的质量和产量造成巨大的损失。目前对于黑斑病的研究在大田防治方面有了较多的研究，但对甘薯黑斑病病菌的生理特征和致病机理的研究不足。本文考察了 49 个甘薯品种对黑斑病菌的抵抗能力，通过高通量测序技术对其中强、弱抗性的品种进行取样、转录组测序。本研究分别根据侵染病斑的深度与表面直径这两个指标来研究它们的基因表达谱差异，通过高通量测序，分别鉴定到了 8 和 19 个显著差异表达基因。本研究通过现代生物技术研究了甘薯响应黑斑病菌的分子基础，鉴定到了一些候选基因，为日后验证和进一步研究这些基因、进而针对黑斑病进行分子育种提供了基础。 50758
毕业论文关键词：甘薯黑斑病抗性分子标记高通量测序转录组测序
Molecular Mechanism in Response to the Black Rot Infection in Sweet Potato (Ipomoea batatas) by Using Transcriptome Analysis
Abstract Sweet potato is one of the most important crops and materials in industry. The black spot disease is one of the most serious threatens in sweet potato, which causes a huge lose to sweet potato’s quality and yield every year. At present, there are a lot of studies of field control for black spot disease, but few studies on its physiological characteristics and molecular mechanisms. The samples of strong and weak resistant varieties were sequenced by high-throughput sequencing to investigate sweet potato varieties’ resistance ability to black spot. This study is according to the depth and diameter of the infection disease to research their differential genes expression. We identifed 8 and 19 significantly differential expressed genes by high-throughput sequencing. This study uses the modern biological technology to find out the molecular basis of sweet potato black spot and have identified some candidate genes, which will provide the foundation for validating and further studying for these genes and then carrying out molecular breeding aiming to black spot.
Key words: Sweet potato black rot resistance, Molecular marker, High throughput sequencing, transcription sequencing

摘要 . 1

Abstract . 1

目录 . 3

图清单 4

表清单 4

1 绪论 5

1.1 甘薯黑斑病病菌致病机理. 5

1.2 甘薯黑斑病的防治. 6

1.3 转录组测序概念及其过程. 6

2 材料与方法 .. 7

2.1 供试材料.. 7

2.1.1鉴定材料的繁殖 7

2.1.2菌液的制备 .. 7

2.2 接种方法.. 8

2.2.1接种工具的准备 8

2.2.2接种操作过程 . 8

2.3 转录组测序. 8

2.3.1RNA的提取 . 8

2.3.2cDNA 的制备与测序 .. 8

2.3.3数据处理 9

2.3.4从头组装 9

2.3.5基因注释 9

2.4 基因差异性分析.. 9

3 结果与分析 .. 10

3.1 极端数据结果与分析. 10

3.2 黑斑病相关抗性基因差异性分析.. 12

3.2.1DepthMean.r性状差异性分析结果 .. 12

3.2.2DiameterMean.r性状差异性分析结果 16

4 讨论 .. 20 利用转录组学研究甘薯响应黑斑病的分子机制:http://www.youerw.com/shengwu/lunwen_54160.html