摘要：绿色植物叶片在日光作用下会进行同化作用，与此同时碳和氮就以糖和蛋白质的形式保存了下来。而影响糖和蛋白质积累有许多的因素，包括温度 光照度强度和湿度等。本文选择了连续三天相同的五个时段来研究同一株三种不同植物的叶片光合作用固定碳氮的效果。三种植物则是同为灌木的常绿植物，它们分别是金边大叶黄杨，栀子和椤木石楠。
    在使用仪器CI-340便携式光合测定仪对三种植物一天五个点测定，让机器自动记录下光照强度和光合作用速率等数据后，立即采集叶片然后到实验室烘干，让干叶片保持当日的糖分和蛋白质含量。在完成室外实验后，在室内用分光光度计和凯氏定氮仪来测量精确的叶片碳氮含量。8929
通过分析光合测定仪的数值，分光光度计和凯氏定氮仪测定出的碳氮含量的数据，来确定怎样的条件可以让植物叶片更快更高效的固定碳元素和氮元素。
关键字：光合作用日变化，碳氮含量测定，光强度。
Compare the daily change of photosynthesis and contents of carbon and nitrogen with 3 kinds of plants
Abstract: Green Plants leaves will doing assimilation under the sunlight, meanwhile the carbon and nitrogen is preserved in the form of sugar and protein. And there are many factors that affect protein and sugar, including temperature, light intensity and humidity etc. This paper chose three days in a row, the same five period of time to study the same plant in three different kind of leaf photosynthesis, And how to fixed carbon and nitrogen. Three plants are evergreen shrub, They are Euonymus Japonicus cv.Aureo-ma, Fructus Gardeniae and photinia dacidsoniae.
      Use the instrument CI-340 portable photosynthesis apparatus for the five point in three days measurement, let the machine record the data of light intensity and photosynthesis rate automatically, collecting the leaves and then dry them immediately in the laboratory. Keep the sugar and protein content on the day. When the outdoor experiment was finished, we use the spectrophotometer and kjeldahi nitrogen determination apparatus to measure the precise content of leaf carbon and nitrogen.
Through the analysis of the numerical value of photosynthetic apparatus spectrophotometer and Kjeldahi nitrogen determination apparatus for the content data of carbon and nitrogen to determine what kind of conditions can make faster and more efficient plant leaf fixed carbon and nitrogen.
Key words: Daily photosynthesis. C/N content determination. The light intensity
目录
第一章    绪论    1
1.1 课题背景    1
1.2研究概况、现状及其发展    1
1.3本文的主要目的和内容    3
第二章：研究内容和实验方法    3
2.1 实验材料与处理    3
2．1.1 实验材料与实验条件    3
2.1.2 实验时间及地点    3
2．1.3 实验主要应用仪器    3
2.2实验方案及测定方法    4
2．2.1 叶片光合作用测定    4
2.2.2 叶绿素的提取和含量测定    4
2.2.3蒽酮法测量叶片糖和淀粉的含量    4
2.2.4 氮的测定    5
第三章 结果与分析    6
3.1植物叶片光合作用数据    6
3.1.1 三天光合有效辐射平均值    6
3.1.2三种植物净光合作用速率数据    6
3.1.3 三植物日固碳量平均值    8
3.1.4 光和数据分析    8
3.2 植物叶片叶绿素含量的测定    9
3.3 植物叶片可溶性糖和淀粉的含量    10 三种植物叶片光合作用日变化及碳氮含量比较研究:http://www.youerw.com/shengwu/lunwen_7468.html