摘要：本论文以我国丰富而又急待开发的葵花籽粕为研究对象对葵花籽粕资源的综合利用进行了较为系统的研究，主要研究了葵花籽粕中富含的活性物质绿原酸的分离工艺以及蛋白质的酶解，为综合利用葵花籽粕资源打下了基础。23459
采用乙醇超声提取法提取葵花粕中的绿原酸，探讨乙醇溶液体积分数，料液比，提取温度对绿原酸提取量的影响，采用正交试验对绿原酸工艺进行优化。结果表明：绿原酸的最佳提取工艺条件为乙醇溶液体积分数50%,提取时间1h，料液比1:10（g/ml），提取1次。
正交试验显示：葵花粕绿原酸的最佳提取条件为提取温度60 ℃、乙醇溶液体积分数55% 、提取时间1.5 h、料液比1:12、pH=6、提取1次。
以物理去油后的葵花籽粕为原料，以水解度为指标，采用单因素实验，选定料液比、酶用量和酶解时间三个因素研究了木瓜蛋白酶以及胰蛋白酶对葵花籽粕的酶解过程，比较各蛋白酶的水解效果得出了各酶水解葵花籽粕的较佳条件。论文网
木瓜性蛋白酶酶解的较佳条件为：酶解pH=6.0，温度60 ℃，底物浓度3%，酶添加量3.0%，酶解时间4 h；胰蛋白酶酶解的较佳条件为：酶解pH=7.5，温度为40 ℃，底物浓度3%，酶添加量4.0%，酶解时间4 h。
毕业论文关键词：绿原酸；超声浸提；木瓜蛋白酶；胰蛋白酶；水解度
Comprehensive utilization of sunflower seed meal
Abstract :The paper focused on sunflower seed meal, which was abundant but not well developed in China. Recently, chlorogenic acid. a component in sunflower seed meal. has been indicated to be bioactive in preventing free radical change and inhibiting cancer growth. Therefore chlorogenic acid was mostly investigated while the comprehensive utilization of sunflower seed meal was systematically studied.
Chlorogenic acid was extracted from sunflower meal using the method of ethanol ultrasonic extraction, and the effect of volume fraction of ethanol solution, solid-liquid ratio, extraction time and the extraction temperature on chlorogenic acid content were disscussed, by the orthogonal test. The results showed that: the optimal extraction conditions of chlorogenic acid was : ethanol solution volume fraction 50%, extraction temperature 50℃, extraction time 1h, ratio of solid / liquid 1:10 (g/ml), extracting 1 time.
Orthogonal test showed that: the optimal extraction conditions of chlorogenic acid of sunflower meal was: extraction temperature 60 ℃, the volume fraction of ethanol solution 55% , extraction time 1.5 h, ratio of solid / liquid 1:12, pH =6, extracting 1 time.
The raw material was the sunflower seed meal that was expressed by physical method, with the degree of hydrolysis as the index, using the single factor experiment. The three factors (selected material / liquid ratio, enzyme dosage and hydrolysis time) of sunflower seed meal enzymolysis hydrolyzed by Papain or Trypsin were analyzed, the optimal extraction conditions of the proteases were obtained by the comparison of effect of hydrolysis.
The optimal extraction conditions of Papain was: Ph=6, temperature 60℃, substrate concentration 3%, enzyme dosage 3%, hydrolysis time 4 h; The optimal extraction conditions of Trypsin was: Ph=7.5, temperature 40℃, substrate concentration 3%, enzyme dosage 4%, hydrolysis time 4 h.
Keywords: Chlorogenic acid; Ultrasonic extraction; Papain ; Trypsin; Degree of hydrolysis
目录
1. 引言    1
1.1 葵花籽概况    1
1.2 葵花籽的应用    1
    1.2.1 葵花籽油的提取    1
    1.2.2 葵花籽饼粕的利用    2
    1.2.3 蛋白质酶解的应用    2
    1.2.4 葵花籽绿原酸的提取的应用    4
1.3国内外研究现状    6 葵花籽粕的综合利用+文献综述:http://www.youerw.com/shiping/lunwen_16525.html