叶面喷施二氢卟吩铁对盐胁迫大豆幼苗的生理缓解效应研究
毕业论文关键词：大豆；二氢卟吩铁；生理效应；盐胁迫
Effects of foliar spraying of DHFe on physiological mitigation of soybean seedlings under salt stress
Abstract：Salt stress is a major abiotic stress that limits crop growth and yield. In this experiment, soybean varieties Lee68 (salt tolerant) and Jackson (salt sensitive) with the different salt tolerance as the experimental materials were sprayed on salt-stressed, soybean seedlings with DHFe. The phenotypes, plant height, osmotic potential, photosynthetic parameters and antioxidant enzyme activities were determined. The results indicated that under the salt stress, the phenotypic variation, plant height, leaf photosynthetic rate(Pn), stomatal conductance(Gs), intercellular CO2 concentration(Ci) and transpiration rate(Tr) of Jackson and Lee68 decreased significantly, however the superoxide anion (O2.-) and H2O2 content in the leaves evidently increased. While the catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) and superoxide dismutase (SOD) activity significantly decreased in the Jackson’s leaves of soybean seedlings, these results were opposite to Lee68. After spraying DHFe, the above indexes of two kinds of soybean seedlings of salt stress suppression were all restored, and the recovery effect of Jackson was relatively obvious. This paper provides the important theoretical basis for the use of DHFe as plant growth and tolerance regulators in the resistance to chemical regulation of soybean and other crops.
Key words: Soybean; DHFe; Physiological effect; Salt stress
目 录
摘要1
关键词1
Abstract1
Key words2
引言2
1材料与方法2
1.1实验材料与培养方法2
1.2大豆形态学观察3
1.3大豆渗透势测定3
1.4大豆光合参数测定3
1.5大豆H2O2含量的测定3
1.6大豆超氧阴离子产生速率的测定4
1.7大豆叶片活性氧酶活性测定4
1.8数据分析5
2结论与分析6
2.1对大豆表型影响6
2.2对大豆渗透势影响6
2.3对大豆光合参数影响7
2.4对过氧化氢含量和超氧阴离子产生速率的影响8
2.5对大豆抗氧化酶系统活性影响8
3讨论 9
致谢10
参考文献10
叶面喷施二氢卟吩铁对盐胁迫大豆幼苗的生理缓解效应研究
引言：
目前全球的土地盐渍化十分严重，其土壤的含盐量超过0.3%，许多植物由于土壤盐渍化问题受到影响，其严重限制作物的生长和产量，是全世界农业面临的一个日益严重的问题[1-4]。在盐胁迫的环境下，迫使植物体内会产生很多自身本该不必要的有机酸，并从植物根部分泌到土壤中，并与植物根部周围土壤中的碱进行中和反应，从而减少了植物根部对于碱性离子的吸收。盐胁迫可导致植物体内的活性氧（ROS）离子的应激反应，渗透胁迫，营养不平衡和氧化应激，并可诱导几种形态的生理和代谢反应[5-7]。盐胁迫会导致细胞内离子含量如Na+和Cl-的增加，以及细胞ROS水平的增加，例如超氧阴离子（O2.-）和过氧化氢（H2O2）。这些盐胁迫和ROS离子能够通过加剧膜脂质过氧化的作用，导致细胞膜通的透性受到损伤以及植株的生理过程，特别是能够通过光合作用来抑制植物生长[5,8,9]。