长期杂草控制对稻茬麦田AMF群落结构的影响
毕业论文关键词：杂草控制；AMF群落结构；土壤理化性质；稻麦两熟制
Impact of Long-term Weed Control on AMF Community Structure in Wheat Field after Rice Reaping Student majoring in Agronomy
Abstract： Arbuscular mycorrhizal fungi (AMF) play an important role in agroecosystem. However, little is known about how different weed control methods affect AMF community structure of wheat field after rice reaping. The goal of the study is to test whether weed control will alter AMF community composition and persity of wheat field after rice reaping. The study has been based on rice-wheat cropping system of middle-lower reaches of Yangtze River for a long-term experiment since 1998. Our treatments are (1) No weed treatment plot, manual+ herbicide weeding, irrigation with a net, around the net (WiWaMH), (2) No weed treatment plot, manual+ herbicide weeding, irrigation without a net (MH), (3) Herbicide treatment plot, irrigation with a net (WaH), (4) Manual treatment plot, irrigation with a net (WaM), (5) Control plot, no weeding (CK). AMF community species information was obtained by pyrosequencing, and soil physiochemical properties data were measured. This study had taken multiple comparative analysis, NMDS analysis, RDA analysis and SIMPER analysis to analyze AMF community structure. The results showed that nitrate nitrogen (NO3--N), available phosphorus (AP), water content (WC) and soil organic carbon (SOC) were the main soil physiochemical properties affecting AMF community structure, among which the most important was NO3--N. The application of pesticide was not the main influencing factor in this experiment. The change of AMF community structure was mainly reflected on the species information. Therefore, it was speculated that the structural changes of AMF community might not be directly affected by weed community, but rather the overall impact of the agroecosystem. Key words: Weed Control；AMF Community Structure；Soil Physiochemical Properties；Rice-wheat Cropping System
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1 材料与方法2
1.1 研究地概况2
1.2 田间设计和管理2
1.3 土壤样品采集与处理3
1.4 实验方法3
1.4.1 土壤理化性质测定3
1.4.2 土壤 AMF 群落4
1.5 数据分析4
2 结果与分析4
2.1 土壤理化性质分析4
2.2 AMF 多样性分析6
2.3 NMDS 分析6
2.4 RDA 分析8
2.5 SIMPER 分析9
3 讨论10
致谢11