早期灌喂母源粪菌对新生仔猪肠道菌群发育的影响
毕业论文关键词：粪菌移植；新生仔猪；肠道菌群
Effect of oral maternal fecal microbiota on intestinal flora development of newborn piglets
Abstract：As a treatment, fecal microbiota transplantation has been used in human intestinal disease,but the research on newborn piglets is still limited. This study was conducted by MiSeq high throughput sequencing technology to investigate the effects of oral maternal fecal intervention on intestinal flora in Duroc × Landrace × Large newborn piglets. We chose 14 piglets which had the same mother, were randomly pided into fecal treatment group and control group. The fecal inoculation group was fed with fecal inoculation solution on the 1-5 days after birth (the volume of the solution gradually increased, 2 mL/d in the first three days, 3 mL/d in the later two days). The control group was perfused with equivalent saline. Piglets’ fecal sample were collected at 1, 3, 5, 7, 10, 14, 18 and 22 days old, and sow’s fecal samples were collected at 19, 20, 21, 23 and 26 days after piglets’ birth. High throughput sequencing results are shown that oral administration of maternal fecal fluid could increase the persity of gut microbiota in piglets to some extent. The proportion of Proteobacteria was decreased significantly, while Bacteroidetes gradually became one of the dominant phyla. From 10 days after birth, the sum of the proportion of Firmicutes and Bacteria was about 90%. Meanwhile, the treatment also significantly changed the abundance of some genus, including Holdemanella, uncultured Ruminococcaceae, Prevotellaceae NK3B31 group, Faecalibacterium and Ruminococcaceae UCG-010. During the first 3 days after birth, Oral administration of maternal fecal fluid did not affect the colonization of intestinal microbiota in piglets, maternal bacteria was considered as the main factor in determining the gut bacterial structure. The effect of oral maternal fecal turbid liquid on intestinal flora’s colonization of piglets was for a maximum of 10 to 14 days. And the cluster of intestinal flora of piglets was close to the sows’ in about 22 days after birth.
Key words: fecal microbiota transplantation; newborn piglets; intestinal flora
目 录
摘要3
关键词3
Abstract3
Key words3
前言3
1材料与方法4
1.1试验材料 4
1.2试验方法 4
1.2.1母源粪菌的制备4
1.2.2粪菌干预试验4
1.2.3仔猪粪样采集4
1.2.4粪样菌群的分析4
1.2.4.1菌群DNA的提取4
1.2.4.2细菌16S rRNA基因V3-V4区序列扩增5