摘要目的：探究高压静电喷雾技术制备海藻酸钙磁性微球的工艺，制备载阿霉素海藻酸钙磁性微球并评价其理化性质。方法：采用高压静电喷雾法制备海藻酸钙磁性微球，考察海藻酸钠浓度、氯化钙浓度、四氧化三铁浓度、静电压、流速、针尖到液面距离、针头内径等参数对海藻酸钙磁性微球的影响。使用荧光倒置显微镜对微球进行粒径测量、圆整度观察和不同干燥方法对微球形态的影响观察；微波治疗仪对微球微波照射的影响，以盐酸阿霉素作为模型药物，制备载药微球，并评价载药磁性微球的理化性质如包封率、载药量和体外释放。结果：海藻酸钙磁性微球粒径分布集中于200 µm，最优参数为海藻酸钠浓度为3％、氯化钙浓度6％、四氧化三铁浓度1。5 mg/mL、静电压15 kv、流速2 mL/h、针尖到液面距离3 cm、针头内径180 µm；磁性微球具有较强磁响应；载药磁性微球的包封率和载药量均随着投药量的增加而呈上升趋势，10％投药量时载药微球包封率为74。50 ± 0。49（％），载药量为1。96 ± 0。39（％）；微球中添加了CaCO3再进行载药，相同的投药量，CaCO3载药磁性微球的药物的包封率和载药量均增加；在PBS（pH =7。4）体外释放中，载药微球和CaCO3载药磁性微球均能缓释达10天，但后者释放较缓慢。结论：通过对参数的优化，可获得形态圆整、粒径为200 µm、具有磁响应、较好的药物包载性能并具有缓释效果的阿霉素海藻酸钙磁性微球。88190

Abstract Objective To explore the preparation of calcium alginate magnetic microspheres by high voltage electrostatic spraying method, and to fabricate and evaluate calcium alginate microspheresof loading doxorubicin。 Methods The factors influencing properties of calcium alginate magnetic microspheres were investigated by high voltage electrostatic spraying method, including sodium alginate concentration, calcium chloride concentration, the content of magnetic oxide, voltage, flow rate,distance between needle and calcium chloride solution,and internal diameter of needle on the magnetic microspheres。 The different drying methods effecting on the morphology of microspheres, the particle size and the roundness were observed by fluorescent inverted microscope。The effect of microwave irradiation on microspheres was investigated by microwave therapy apparatus。 The drug-loaded  microspheres were prepared by using doxorubicin hydrochloride as the model drug, and the properties of the drug-loaded magnetic microspheres were evaluated, such as entrapment efficiency, drug loading and in vitro releasediamete。 Results The size distribution of calcium alginate magnetic microspheres was concentrated at 200μm。 The optimum parameters were 3% of sodium alginate, 6% of calcium chloride concentration, 1。5 mg / mL of magnetic oxide, 15 kv of voltage and 2 mL /h of flow rate, the distance between needle and calcium chloride solution of 3cm, internal diameter of 180 µm。 The encapsulation efficiency and drug loading of magnetic microspheres were increased with the increase of dosage, and when 10% doxorubicin hydrochloride was added，the encapsulation efficiency and drug loading of microspheres were 74。50 ± 0。49 (%) and 1。96 ± 0。39 (%)。With the addition of CaCO3 into magnetic microspheres, the increased encapsulation efficiency and drug loading of microspheres were found。 With PBS（pH =7。4）  as medium, drug release from microspheres and CaCO3-loaded magnetic microspheres lasted for 10 days in vitro, but the latter release was slower。 Conclusion By optimizing the parameters, the adriamycin calcium alginate magnetic microspheres with the shape roundness, the particle size of 200 μm, the magnetic response, the better drug loading performance and the sustained release effect were obtained。

毕业论文关键词：磁性微球； 阿霉素； 高压静电喷雾法；体外释放