摘要：为考查氢溴酸常山酮预混剂在靶动物鸡上应用的安全性，本试验以推荐剂量的1、3、5倍剂量给药，饲喂42天，通过对鸡的增重情况、血液学与血液化学指标及组织病理学检查，了解受试药物在鸡中的剂量-反应曲线，即从有效剂量到毒性作用；了解鸡对药物中毒剂量的临床反应特征；了解受试药物有效剂量、推荐剂量和中毒剂量对靶动物的组织病理学和生理生化指标影响的变化特征，从而评价氢溴酸常山酮对鸡的安全性。试验结果表明，各试验组与对照组的血液化学指标和血液学指标个别有显著差异，但总体在参考范围内，尸体解剖未见各组织器官有明显的异常变化，组织学检查也未见有明显病理变化，但当饲料中的药物浓度超过一定浓度时会影响鸡只采食和鸡日增重，甚至会使鸡只行为异常，因此在临床应用时应主要混合均匀度问题。29824
毕业论文关键词：氢溴酸常山酮；安全性；鸡；靶动物。
Evaluation of the safety of Halofuginone Hydrobromide premixes on chickens
Abstract：To evaluate the safety of halofuginone hydrobromide pre-blends in target animal chickens,the experiment was administered at a recommended dose of 1,3,5 times for 42 days.Through the weight gain of the chicken,hematology and hematology and histopathological examination to understand the dose-response curve of the test drug in chickens, from effective dose to toxic effects; to understand the clinical response characteristics of the drug dose of chicken; to understand the effective dose of the test drug , Recommended dose and poisoning dose on the target animal pathology and physiological and biochemical indicators of the changes in characteristics, to evaluate the halofuginone hydrobromide on chicken safety. The results showed that there were no significant differences in hematological and hematological indexes between the experimental group and the control group. There were no obvious abnormal changes in the organs and organs of the autopsy. There was no pathological changes in histological examination, However, when the concentration of drug in the feed exceeds a certain concentration, it will affect the daily feeding of chickens and the daily gain of chickens, even can make chickens behave differently.Therefore, it is necessary to mix the homogeneity evenly in clinical application.
Key words: Halofuginone hydrobromide ;Safety;Chicken;Target animal.
目  录

摘要1
关键词1
Abstract1
Key words1
引言1
1材料与方法1
1.1试验材料 2
1.1.1试验动物 2
1.1.2试验药物2
1.1.3仪器设备2
1.2实验方案2
1.2.1试验方法2
1.2.2样品采集与处理2
1.2.3观察指标2
1.2.4统计分析 3
2结果与分析 3
2.1临床观察3
2.2体重变化与采食量 3
2.3血液常规指标分析4
2.4血液生化指标分析6
2.5脏器系数8
2.6解剖学与组织学观察9
3讨论12
致谢13
参考文献13
氢溴酸常山酮预混剂对鸡的安全性评价
引言
鸡球虫是严重的禽病之一[1]，常感染雏鸡，严重时可使鸡血便，出血甚至大量死亡，据估计，每年损失可达20亿英镑[2]。氢溴酸常山酮是由美国氰胺公司于1964年人工合成的，由于氢溴酸常山酮广谱低毒，无交叉耐药，上市后被大量使用[3]。虽然该药具有广阔的市场，但在其长期使用的安全性却没有文献报道。前期试验证明，该药抗球虫指数高达以上，具有高效抗球虫效果[4][5]。为了进一步掌握该药物的各项数据，做好深入研究其作用机理前的准备工作，并为临床合理用药提供指导，从而提出受试药物的不良反应、防治措施及临床应用时的注意事项。
1 材料与方法
1.1试验材料
1.1.1试验动物 氢溴酸常山酮预混剂对鸡的安全性评价:http://www.youerw.com/yixue/lunwen_25221.html