摘要帕金森病是一种常见的慢性神经退行性疾病，其特点是脑黑质致密部的多巴胺能神经元选择性的缺失和死亡，导致运动障碍，如动作迟缓、手脚震颤等等。全世界60岁以上人群帕金森病的发病率高达1%。而发病原因尚未可知，但已有研究表明遗传、免疫异常、衰老、环境毒素等因素都会引起帕金森病。斑马鱼是当今在人类疾病模型中新型的模式动物，非常适用于构建帕金森疾病模型。本文在氧化应激实验模型的基础上，探究的是野生型斑马鱼中脑多巴胺神经元数量与其行为学相关性。实验表明多巴胺神经元的少量缺失会引起行为学上的极度活跃，而过度缺失会导致活动衰减，甚至运动障碍。43965

Abstract Parkinson’s disease(PD) is a chronic neurodegenerative disease with symptoms that include bradykinesia, akinesia, rigidity and tremor, due to degeneration of dopaminergic neurons in substantia nigra and decrease of dopamine content in striatum. The incidence of PD is about 1% in aged person over 60 in the world. But the causes of PD are still unknown. The relevant studies have shown that genetic factors, infections, immunological abnormalities, aging, toxins(endogenous and exogenous) may all involve in the development of PD. Zebrafish as a new model animal for human disease model, is applicable to PD model. This composition will state preliminary study on the relationship between the number of midbrain dopaminergic neurons and behavior in wild type zebrafish, based on oxidative stress experimental model. Experiments showed that mild DA reduction can lead to hyperactivity while severe DA reduction can lead to hypoactivity, even dyskinesia.

毕业论文关键词：帕金森病； 斑马鱼； 疾病模型；多巴胺神经元

Keyword: Parkinson’s disease; zebrafish; disease model; dopaminergic neuron

目   录 

摘要 Ⅰ

目录 Ⅱ

1　引言 1

1.1　帕金森病概述 1

1.1.1　帕金森病的症状 1

1.1.2　帕金森病的致病机理 1

1.2　帕金森病医疗现状 2

1.2.1　帕金森病在国际上的医疗情况 2

1.2.2　帕金森病的中医诊治 2

1.3　斑马鱼疾病模型概述 3

1.3.1　斑马鱼作为模式动物的生理学特性 3

1.3.2　斑马鱼疾病模型的应用 3

2　课题简介 4

2.1　目的及意义 4

2.2　研究内容 5

3　材料和方法 5

3.1　DAT:EGFP转基因斑马鱼 5

3.2　斑马鱼饲养及胚胎收集 5

3.3　试剂和溶液配方 5

3.4　实验方法 7

3.4.1　斑马鱼的基因型鉴定 7

3.4.1.1　碱裂解法提取DNA 7

3.4.1.2　聚合酶链式反应 8

3.4.1.3　琼脂糖凝胶电泳 9

3.4.2　氧化应激模型实验 9

3.4.3　行为学分析实验 9

3.4.4　共聚焦显微镜观测多巴胺神经元实验 野生型斑马鱼中脑多巴胺神经元数量与其行为学相关性的初步研究:http://www.youerw.com/yixue/lunwen_45086.html