可注射微球复合水凝胶型骨修复支架的制备

摘要软骨组织工程化软骨组织生长所依赖的合适的环境由细胞骨架提供。水凝胶材料是在结构上与基质和外天然软骨细胞非常相似，并且可以通过一针法注入来实现软骨组织工程的微创治疗，有效地减少了对病人的手术的负担，是用于软骨修复一个类型非常有前景的材料。微球材料具有更好的持续药物释放，可以调节药物延长功效的释放速率，降低的毒性减少给药频率，包埋药物，从而避免失活，而微球还可以嵌入在细胞生长因子和种植细胞等。在本文中，利用席夫碱反应制备可注射海藻酸钠-壳聚糖水凝胶，采用乳化热交联法制备包载牛血清蛋白的PLGA微球，并将载有牛血清蛋白的PLGA微球加入水凝胶中，将水凝胶的可注射性与PLGA微球的药物缓释作用相结合，获得了一种可注射型软骨修复复合水凝胶支架，并研究了该复合水凝胶的性能与结构。87815

毕业论文关键词 PLGA微球可注射型水凝胶复合支架软骨修复

毕业设计说明书外文摘要

Title Construction of PLGA hydrogel composite system and Drug delivery propeties

Abstract In cartilage tissue engineering, the suitable environment for the growth of cartilage tissue is provided by the cell scaffold。Hydrogels in structure and natural cartilage extracellular matrix is very similar, and through the injection needle method to achieve minimally invasive treatment for cartilage tissue engineering, effectively reducing the burden to the patients and is used for cartilage repair a very promising materials。Microspheres exhibit good sustained-release effect, regulating drug release rate extend the efficacy, reduce dosing frequency to reduce toxic and side effects, drug embedding and thus avoid inactivation and microspheres can also be entrapped cell growth factor and plant cells。In this paper, the injectable alginate chitosan hydrogel was prepared by using the reaction of Schiff Base reaction,using emulsion crosslinking method preparation of bovine serum protein loaded PLGA microspheres, and loaded with PLGA microspheres containing bovine serum albumin added to the hydrogel, of PLGA microspheres sustained release of the drug action and hydrogel injection combined won the a can injectable cartilage composite hydrogel scaffolds to repair, and to study the structure and properties of the composite hydrogels。

Keywords PLGA microspheres Injectable hydroge composite systems cartilage repair

1 绪论 1

1。1 软骨组织工程 1

1。2 微球 1

1。2。1 微球定义及特性 1

1。2。2 PLGA微球 2

1。2。3 微球的制备方法 2

1。3 水凝胶 3

1。3。1 水凝胶的分类 3

1。3。2 水凝胶的制备 4

1。4 海藻酸钠和壳聚糖 5

1。4。1 海藻酸钠 5源-于,优Y尔E论W文.网wwW.yOueRw.com 原文+QQ752018`766

1。4。2 壳聚糖 5

1。5 课题的提出 5

2。1实验材料与仪器 7

2。2实验方法 8

2。2。1 空白PLGA微球的制备可注射微球复合水凝胶型骨修复支架的制备:http://www.youerw.com/cailiao/lunwen_146871.html