摘要介孔纳米硅球是一种新型的纳米有序介孔材料,它具有比表面积大,孔道大小可调节,排序均匀,有良好的生物相容性等优点,它可以被填入不同的小分子并制成不同响应的探针,在构建胞内生物转运平台上表现出了巨大的发展潜力。本次研究的一系列工作包括用改良的溶胶-凝胶法合成介孔纳米硅球并将其中填入荧光剂,黑洞猝灭剂并用DNA序列作为门控,制备成了DNA响应的对CEA,PSA等肿瘤标志物具有特异性响应的分子探针。最后,本次实验还对该探针的性能做了研究,对该探针的发展和改进提出了一系列建议。
关键词 介孔纳米硅球 荧光 特异识别 肿瘤标志物47862
毕业论文设计说明书外文摘要
Title Research on the synthesis of mesoporous silica nanoparticles and the controlled release of fluorochrome
Abstract
Mesoporous silica nanoparticles is a new kind of ordered mesoporous nanoscale materials. Due to its characteristics of having great specific surface area, size-adjustable and well ordered pore,and good biocompatibility, it can be filled with different small molecules and be made into probe with perse specific response, which shows great potential in the establishment of intracellular biological operation platform. This research included the synthesis of Mesoporous silica nanoparticles through modified sol-gel method, filling it with fluorochrome as well as BHQ-1 carboxylic acid the and then using the homologous DNA as the gating. Finally we got pore responses to CEA, PSA and other tumor marker. Then i researched on the performance of the probe. Based on the research, I gave some advice on the development and improvement of the probe.
Keywords Mesoporous silica nanoparticles tumor biomarker fluorochrome specific response
目 次
1 引言 1
2 实验部分 4
2.1 实验的研究内容、研究目标 4
2.1.1 功能性多孔硅球的合成 4
2.1.2 染料的可控释放研究 4
2.1.3 实验的研究目标 4
2.2 具体实验部分 4
2.2.1 实验所需的设备,材料与试剂 5
2.2.2 多孔硅纳米粒子的合成 5
2.2.3 多孔硅纳米粒子的丙氨基功能化 5
2.2.4 荧光素的填充 6
2.2.5 探针的制备 6
2.2.6 多孔硅纳米粒子的合成表征 7
2.2.7 以核酸适配体作为门控研究染料的封堵与释放 7
3 实验结果分析与讨论 8
3.1 多孔硅纳米粒子的合成表征结果和讨论 8
3.2 染料可控释放的研究结果(基于肿瘤标志物响应的控释体系的探究结果) 9
3.2.1 填充异硫氰酸荧光素(FITC),包裹CEA-apt的探针的控释体系的研究 9
3.2.2 填充罗丹明B,包裹PSA-apt的探针的控释体系的研究 12
3.2.2 填充异硫氰酸荧光素(FITC),包裹AFP-apt的探针的控释体系的研究 多孔硅球合成和染料可控释放研究:http://www.youerw.com/huaxue/lunwen_50126.html